Leucine-rich glioma inactivated 3 is a melanogenic cytokine in human skin

Hyo Soon Jeong, Yun Mi Jeong, Jandi Kim, Seung Hoon Lee, Hye Ryung Choi, Kyungchan Park, Beom Joon Kim, Kwang Jin Baek, Nyoun Soo Kwon, Hye Young Yun, Dong Seok Kim

Research output: Contribution to journalLetter

9 Citations (Scopus)

Abstract

Recently, we demonstrated that leucine-rich glioma inactivated 3 (LGI3) is expressed in human skin. However, the effects of LGI3 on melanocytes remain unknown. The present study demonstrated that LGI3 can serve to stimulate melanogenesis without affecting cell viability. To determine the effects of LGI3 on melanin synthesis, normal human melanocytes and Mel-Ab cells were treated with recombinant LGI3 and melanin content was measured. Our results showed that LGI3 promoted melanin synthesis in both cell types. Moreover, upregulation of microphthalmia-associated transcription factor (MITF) and tyrosinase was observed at both the mRNA and protein levels via RT-PCR and Western blotting, respectively. Furthermore, immunohistochemical staining showed that the expression of LGI3 increased in the basal layer of melasma skin samples, whereas it decreased slightly in vitiligo samples. These results suggest that LGI3 may play a role as a melanogenic cytokine in human skin.

Original languageEnglish
Pages (from-to)600-602
Number of pages3
JournalExperimental Dermatology
Volume23
Issue number8
DOIs
StatePublished - 1 Jan 2014

Fingerprint

Leucine
Glioma
Skin
Cytokines
Melanins
Melanocytes
Microphthalmia-Associated Transcription Factor
Melanosis
Vitiligo
Monophenol Monooxygenase
Cell Survival
Up-Regulation
Western Blotting
Cells
Staining and Labeling
Polymerase Chain Reaction
Messenger RNA
Proteins

Keywords

  • LGI3
  • MITF
  • Melanin
  • Melanogenesis
  • Tyrosinase

Cite this

Jeong, H. S., Jeong, Y. M., Kim, J., Lee, S. H., Choi, H. R., Park, K., ... Kim, D. S. (2014). Leucine-rich glioma inactivated 3 is a melanogenic cytokine in human skin. Experimental Dermatology, 23(8), 600-602. https://doi.org/10.1111/exd.12462
Jeong, Hyo Soon ; Jeong, Yun Mi ; Kim, Jandi ; Lee, Seung Hoon ; Choi, Hye Ryung ; Park, Kyungchan ; Kim, Beom Joon ; Baek, Kwang Jin ; Kwon, Nyoun Soo ; Yun, Hye Young ; Kim, Dong Seok. / Leucine-rich glioma inactivated 3 is a melanogenic cytokine in human skin. In: Experimental Dermatology. 2014 ; Vol. 23, No. 8. pp. 600-602.
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title = "Leucine-rich glioma inactivated 3 is a melanogenic cytokine in human skin",
abstract = "Recently, we demonstrated that leucine-rich glioma inactivated 3 (LGI3) is expressed in human skin. However, the effects of LGI3 on melanocytes remain unknown. The present study demonstrated that LGI3 can serve to stimulate melanogenesis without affecting cell viability. To determine the effects of LGI3 on melanin synthesis, normal human melanocytes and Mel-Ab cells were treated with recombinant LGI3 and melanin content was measured. Our results showed that LGI3 promoted melanin synthesis in both cell types. Moreover, upregulation of microphthalmia-associated transcription factor (MITF) and tyrosinase was observed at both the mRNA and protein levels via RT-PCR and Western blotting, respectively. Furthermore, immunohistochemical staining showed that the expression of LGI3 increased in the basal layer of melasma skin samples, whereas it decreased slightly in vitiligo samples. These results suggest that LGI3 may play a role as a melanogenic cytokine in human skin.",
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Jeong, HS, Jeong, YM, Kim, J, Lee, SH, Choi, HR, Park, K, Kim, BJ, Baek, KJ, Kwon, NS, Yun, HY & Kim, DS 2014, 'Leucine-rich glioma inactivated 3 is a melanogenic cytokine in human skin', Experimental Dermatology, vol. 23, no. 8, pp. 600-602. https://doi.org/10.1111/exd.12462

Leucine-rich glioma inactivated 3 is a melanogenic cytokine in human skin. / Jeong, Hyo Soon; Jeong, Yun Mi; Kim, Jandi; Lee, Seung Hoon; Choi, Hye Ryung; Park, Kyungchan; Kim, Beom Joon; Baek, Kwang Jin; Kwon, Nyoun Soo; Yun, Hye Young; Kim, Dong Seok.

In: Experimental Dermatology, Vol. 23, No. 8, 01.01.2014, p. 600-602.

Research output: Contribution to journalLetter

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T1 - Leucine-rich glioma inactivated 3 is a melanogenic cytokine in human skin

AU - Jeong, Hyo Soon

AU - Jeong, Yun Mi

AU - Kim, Jandi

AU - Lee, Seung Hoon

AU - Choi, Hye Ryung

AU - Park, Kyungchan

AU - Kim, Beom Joon

AU - Baek, Kwang Jin

AU - Kwon, Nyoun Soo

AU - Yun, Hye Young

AU - Kim, Dong Seok

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Recently, we demonstrated that leucine-rich glioma inactivated 3 (LGI3) is expressed in human skin. However, the effects of LGI3 on melanocytes remain unknown. The present study demonstrated that LGI3 can serve to stimulate melanogenesis without affecting cell viability. To determine the effects of LGI3 on melanin synthesis, normal human melanocytes and Mel-Ab cells were treated with recombinant LGI3 and melanin content was measured. Our results showed that LGI3 promoted melanin synthesis in both cell types. Moreover, upregulation of microphthalmia-associated transcription factor (MITF) and tyrosinase was observed at both the mRNA and protein levels via RT-PCR and Western blotting, respectively. Furthermore, immunohistochemical staining showed that the expression of LGI3 increased in the basal layer of melasma skin samples, whereas it decreased slightly in vitiligo samples. These results suggest that LGI3 may play a role as a melanogenic cytokine in human skin.

AB - Recently, we demonstrated that leucine-rich glioma inactivated 3 (LGI3) is expressed in human skin. However, the effects of LGI3 on melanocytes remain unknown. The present study demonstrated that LGI3 can serve to stimulate melanogenesis without affecting cell viability. To determine the effects of LGI3 on melanin synthesis, normal human melanocytes and Mel-Ab cells were treated with recombinant LGI3 and melanin content was measured. Our results showed that LGI3 promoted melanin synthesis in both cell types. Moreover, upregulation of microphthalmia-associated transcription factor (MITF) and tyrosinase was observed at both the mRNA and protein levels via RT-PCR and Western blotting, respectively. Furthermore, immunohistochemical staining showed that the expression of LGI3 increased in the basal layer of melasma skin samples, whereas it decreased slightly in vitiligo samples. These results suggest that LGI3 may play a role as a melanogenic cytokine in human skin.

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