Abstract

During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.

Original languageEnglish
Pages (from-to)315-320
Number of pages6
JournalJournal of Korean Medical Science
Volume31
Issue number2
DOIs
StatePublished - 1 Jan 2016

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Disease Outbreaks
Vero Cells
Korea
Transmission Electron Microscopy
Virion
Cell Culture Techniques
Genome
Viruses
Middle East Respiratory Syndrome Coronavirus

Keywords

  • Korea
  • Microscopy, electron
  • Middle east respiratory syndrome
  • Middle east respiratory syndrome coronavirus
  • Phylogeny

Cite this

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title = "Isolation of middle east respiratory syndrome coronavirus from a patient of the 2015 Korean outbreak",
abstract = "During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.",
keywords = "Korea, Microscopy, electron, Middle east respiratory syndrome, Middle east respiratory syndrome coronavirus, Phylogeny",
author = "Park, {Wan Beom} and Kwon, {Nak Jung} and Choe, {Pyoeng Gyun} and Choi, {Su Jin} and Oh, {Hong Sang} and Lee, {Sang Min} and Hyonyong Chong and Kim, {Jong Il} and Song, {Kyoung Ho} and Bang, {Ji Hwan} and Kim, {Eu Suk} and Kim, {Hong Bin} and Park, {Sang Won} and Kim, {Nam Joong} and Oh, {Myoung Don}",
year = "2016",
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doi = "10.3346/jkms.2016.31.2.315",
language = "English",
volume = "31",
pages = "315--320",
journal = "Journal of Korean medical science",
issn = "1011-8934",
publisher = "Korean Academy of Medical Science",
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TY - JOUR

T1 - Isolation of middle east respiratory syndrome coronavirus from a patient of the 2015 Korean outbreak

AU - Park, Wan Beom

AU - Kwon, Nak Jung

AU - Choe, Pyoeng Gyun

AU - Choi, Su Jin

AU - Oh, Hong Sang

AU - Lee, Sang Min

AU - Chong, Hyonyong

AU - Kim, Jong Il

AU - Song, Kyoung Ho

AU - Bang, Ji Hwan

AU - Kim, Eu Suk

AU - Kim, Hong Bin

AU - Park, Sang Won

AU - Kim, Nam Joong

AU - Oh, Myoung Don

PY - 2016/1/1

Y1 - 2016/1/1

N2 - During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.

AB - During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.

KW - Korea

KW - Microscopy, electron

KW - Middle east respiratory syndrome

KW - Middle east respiratory syndrome coronavirus

KW - Phylogeny

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U2 - 10.3346/jkms.2016.31.2.315

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M3 - Article

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VL - 31

SP - 315

EP - 320

JO - Journal of Korean medical science

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SN - 1011-8934

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ER -