Interleukin-32β Stimulates migration of MDA-MB-231 and MCF-7cells via the VEGF-STAT3 signaling pathway

Jeong Su Park, Su Yun Choi, Jeong Hyung Lee, Maria Lee, Eun Sook Nam, Ae Lee Jeong, Sunyi Lee, Sora Han, Myeong Sok Lee, Jong Seok Lim, Do Young Yoon, Yongil Kwon, Young Yang

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Background: IL-32 is known to play an important role in inflammatory and autoimmune disease responses. In addition to its role in these responses, IL-32 and its different isoforms have in recent years been implicated in the development of various cancers. As of yet, the role of IL-32 in breast cancer has remained largely unknown. Results: By performing immunohistochemical assays on primary breast cancer samples, we found that the level of IL-32β expression was positively correlated with tumor size, number of lymph node metastases and tumor stage. In addition, we found that breast cancer-derived MDA-MB-231 cells exogenously expressing IL-32β exhibited increased migration and invasion capacities. These increased capacities were found to be associated with an increased expression of the epithelial mesenchymal transition (EMT) markers vimentin and Slug, the latter of which is responsible for the increase in vimentin transcription. To next investigate whether IL-32β enhances migration and invasion through a soluble factor, we determined the levels of several migration-stimulating ligands, and found that the production of VEGF was increased by IL-32β. In addition, we found that IL-32β-induced VEGF increased migration and invasion through STAT3 activation. Conclusion: The IL-32β-VEGF-STAT3 pathway represents an additional pathway that mediates the migration and invasion of breast cancer cells under the conditions of normoxia and hypoxia.

Original languageEnglish
Pages (from-to)493-503
Number of pages11
JournalCellular Oncology
Volume36
Issue number6
DOIs
StatePublished - 1 Dec 2013

Keywords

  • Breast cancer
  • Interleukin-32
  • STAT3
  • VEGF

Cite this

Park, J. S., Choi, S. Y., Lee, J. H., Lee, M., Nam, E. S., Jeong, A. L., Lee, S., Han, S., Lee, M. S., Lim, J. S., Yoon, D. Y., Kwon, Y., & Yang, Y. (2013). Interleukin-32β Stimulates migration of MDA-MB-231 and MCF-7cells via the VEGF-STAT3 signaling pathway. Cellular Oncology, 36(6), 493-503. https://doi.org/10.1007/s13402-013-0154-4