Interaction of SPIN90 with dynamin I and its participation in synaptic vesicle endocytosis

Yujin Kim, Sunyun Kim, Suho Lee, Sung Hyun Kim, Yoonju Kim, Zee Yong Park, Woo Keun Song, Sunghoe Chang

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

SH3 protein interacting with Nek, 90 kDa (SPIN90) is an Nek-binding protein that contains one Src homology 3 (SH3) domain, three proline-rich domains (PRDs), a serine/threonine-rich region, and a hydrophobic C-terminal region. Previously, we have shown that SPIN90 plays roles in the sarcomere assembly in cardiac muscles and in the formation of focal contacts in HeLa cells. Besides in heart, SPIN90 is also highly expressed in the brain, but its role in the neuronal system is completely unknown. Here, we found that SPIN90 is expressed in the presynaptic compartment in which it binds dynamin I, a key component of the endocytic machinery, and that it participates in synaptic vesicle endocytosis. Pull-down analysis and coimmunoprecipitation proved the associations of SPIN90 with dynamin I through SH3-PRD interaction. Overexpression of SPIN90 or knocking down SPIN90 by small interfering RNA impaired synaptic vesicle endocytosis. We further confirmed by the rescue experiments that the endocytic defects by SPIN90 expression come from its interaction with dynamin I. Exocytosis kinetics was not affected by SPIN90 expression. Together, our findings suggest that SPIN90 could modulate the interactions of dynamin I with other endocytic proteins that cooperate in the coated vesicle formation, thus regulating synaptic vesicle endocytosis.

Original languageEnglish
Pages (from-to)9515-9523
Number of pages9
JournalJournal of Neuroscience
Volume25
Issue number41
DOIs
StatePublished - 12 Oct 2005

Fingerprint

Dynamin I
Synaptic Vesicles
Endocytosis
Proteins
Proline
Coated Vesicles
Sarcomeres
Focal Adhesions
src Homology Domains
Exocytosis
Threonine
HeLa Cells
Serine
Small Interfering RNA

Keywords

  • Dynamin I
  • Endocytosis
  • PRD
  • SH3
  • SPIN90
  • Synaptic vesicle

Cite this

Kim, Yujin ; Kim, Sunyun ; Lee, Suho ; Kim, Sung Hyun ; Kim, Yoonju ; Park, Zee Yong ; Song, Woo Keun ; Chang, Sunghoe. / Interaction of SPIN90 with dynamin I and its participation in synaptic vesicle endocytosis. In: Journal of Neuroscience. 2005 ; Vol. 25, No. 41. pp. 9515-9523.
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Interaction of SPIN90 with dynamin I and its participation in synaptic vesicle endocytosis. / Kim, Yujin; Kim, Sunyun; Lee, Suho; Kim, Sung Hyun; Kim, Yoonju; Park, Zee Yong; Song, Woo Keun; Chang, Sunghoe.

In: Journal of Neuroscience, Vol. 25, No. 41, 12.10.2005, p. 9515-9523.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Kim, Yujin

AU - Kim, Sunyun

AU - Lee, Suho

AU - Kim, Sung Hyun

AU - Kim, Yoonju

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AU - Song, Woo Keun

AU - Chang, Sunghoe

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