Insulin restores fatty acid composition earlier in liver microsomes than erythrocyte membranes in streptozotocin-induced diabetic rats

Chan Soo Shin, M. K. Lee, Kyong Soo Park, S. Y. Kim, B. Y. Cho, H. K. Lee, C. S. Koh, H. K. Min

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Alterations of fatty acid composition have been observed in a number of tissues in both experimental and human diabetes. Suppression of Δ6 desaturase in the liver, a key enzyme of fatty acid desaturation, has been reported to be responsible for these phenomena. We measured the fatty acid composition of the liver and the erythrocytes, and examined Δ6 desaturase activities to compare the effect of short-term insulin therapy on the tissues with and without Δ6 desaturase, ie., the liver and the erythrocytes using streptozotocin (STZ)-induced diabetic rats. Linoleic (P < 0.05), palmitic (P < 0.01) and docosahexaenoic (DHA) acid (P < 0.01) were higher and arachidonic (P < 0.01) and oleic acid (P < 0.01) were lower in the liver microsomes of diabetic rats when compared to those in control rats. These alterations were partly reversed with insulin treatment. In the erythrocyte membrane, linoleic (P < 0.01) and stearic acid (P < 0.05) were higher, and palmitic (P < 0.05), palmitoleic (P < 0.01), and arachidonic acid (P < 0.01) were lower in diabetic rats. In contrast to the case of the liver microsomes, however, these alterations were persistently observed after 48 h of insulin treatment. The activities of Δ6 desaturase in diabetic rats were 68% of those of controls (P < 0.05), and increased to 119% of controls after insulin treatment. These results show that insulin restores the fatty acid composition earlier in the liver microsome than in the erythrocyte membrane in STZ-induced diabetic rats. The erythrocyte membrane would not be suitable for the investigation dealing with rapid changes of fatty acid composition.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalDiabetes Research and Clinical Practice
Volume29
Issue number2
DOIs
StatePublished - 1 Jan 1995

Fingerprint

Erythrocyte Membrane
Liver Microsomes
Streptozocin
Fatty Acids
Insulin
Liver
Erythrocytes
Docosahexaenoic Acids
Oleic Acid
Cell- and Tissue-Based Therapy
Arachidonic Acid
Therapeutics
Enzymes

Keywords

  • Erythrocyte membrane
  • Fatty acid composition
  • Insulin
  • Microsome
  • Δ6 desaturase

Cite this

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title = "Insulin restores fatty acid composition earlier in liver microsomes than erythrocyte membranes in streptozotocin-induced diabetic rats",
abstract = "Alterations of fatty acid composition have been observed in a number of tissues in both experimental and human diabetes. Suppression of Δ6 desaturase in the liver, a key enzyme of fatty acid desaturation, has been reported to be responsible for these phenomena. We measured the fatty acid composition of the liver and the erythrocytes, and examined Δ6 desaturase activities to compare the effect of short-term insulin therapy on the tissues with and without Δ6 desaturase, ie., the liver and the erythrocytes using streptozotocin (STZ)-induced diabetic rats. Linoleic (P < 0.05), palmitic (P < 0.01) and docosahexaenoic (DHA) acid (P < 0.01) were higher and arachidonic (P < 0.01) and oleic acid (P < 0.01) were lower in the liver microsomes of diabetic rats when compared to those in control rats. These alterations were partly reversed with insulin treatment. In the erythrocyte membrane, linoleic (P < 0.01) and stearic acid (P < 0.05) were higher, and palmitic (P < 0.05), palmitoleic (P < 0.01), and arachidonic acid (P < 0.01) were lower in diabetic rats. In contrast to the case of the liver microsomes, however, these alterations were persistently observed after 48 h of insulin treatment. The activities of Δ6 desaturase in diabetic rats were 68{\%} of those of controls (P < 0.05), and increased to 119{\%} of controls after insulin treatment. These results show that insulin restores the fatty acid composition earlier in the liver microsome than in the erythrocyte membrane in STZ-induced diabetic rats. The erythrocyte membrane would not be suitable for the investigation dealing with rapid changes of fatty acid composition.",
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Insulin restores fatty acid composition earlier in liver microsomes than erythrocyte membranes in streptozotocin-induced diabetic rats. / Shin, Chan Soo; Lee, M. K.; Park, Kyong Soo; Kim, S. Y.; Cho, B. Y.; Lee, H. K.; Koh, C. S.; Min, H. K.

In: Diabetes Research and Clinical Practice, Vol. 29, No. 2, 01.01.1995, p. 93-98.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Insulin restores fatty acid composition earlier in liver microsomes than erythrocyte membranes in streptozotocin-induced diabetic rats

AU - Shin, Chan Soo

AU - Lee, M. K.

AU - Park, Kyong Soo

AU - Kim, S. Y.

AU - Cho, B. Y.

AU - Lee, H. K.

AU - Koh, C. S.

AU - Min, H. K.

PY - 1995/1/1

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N2 - Alterations of fatty acid composition have been observed in a number of tissues in both experimental and human diabetes. Suppression of Δ6 desaturase in the liver, a key enzyme of fatty acid desaturation, has been reported to be responsible for these phenomena. We measured the fatty acid composition of the liver and the erythrocytes, and examined Δ6 desaturase activities to compare the effect of short-term insulin therapy on the tissues with and without Δ6 desaturase, ie., the liver and the erythrocytes using streptozotocin (STZ)-induced diabetic rats. Linoleic (P < 0.05), palmitic (P < 0.01) and docosahexaenoic (DHA) acid (P < 0.01) were higher and arachidonic (P < 0.01) and oleic acid (P < 0.01) were lower in the liver microsomes of diabetic rats when compared to those in control rats. These alterations were partly reversed with insulin treatment. In the erythrocyte membrane, linoleic (P < 0.01) and stearic acid (P < 0.05) were higher, and palmitic (P < 0.05), palmitoleic (P < 0.01), and arachidonic acid (P < 0.01) were lower in diabetic rats. In contrast to the case of the liver microsomes, however, these alterations were persistently observed after 48 h of insulin treatment. The activities of Δ6 desaturase in diabetic rats were 68% of those of controls (P < 0.05), and increased to 119% of controls after insulin treatment. These results show that insulin restores the fatty acid composition earlier in the liver microsome than in the erythrocyte membrane in STZ-induced diabetic rats. The erythrocyte membrane would not be suitable for the investigation dealing with rapid changes of fatty acid composition.

AB - Alterations of fatty acid composition have been observed in a number of tissues in both experimental and human diabetes. Suppression of Δ6 desaturase in the liver, a key enzyme of fatty acid desaturation, has been reported to be responsible for these phenomena. We measured the fatty acid composition of the liver and the erythrocytes, and examined Δ6 desaturase activities to compare the effect of short-term insulin therapy on the tissues with and without Δ6 desaturase, ie., the liver and the erythrocytes using streptozotocin (STZ)-induced diabetic rats. Linoleic (P < 0.05), palmitic (P < 0.01) and docosahexaenoic (DHA) acid (P < 0.01) were higher and arachidonic (P < 0.01) and oleic acid (P < 0.01) were lower in the liver microsomes of diabetic rats when compared to those in control rats. These alterations were partly reversed with insulin treatment. In the erythrocyte membrane, linoleic (P < 0.01) and stearic acid (P < 0.05) were higher, and palmitic (P < 0.05), palmitoleic (P < 0.01), and arachidonic acid (P < 0.01) were lower in diabetic rats. In contrast to the case of the liver microsomes, however, these alterations were persistently observed after 48 h of insulin treatment. The activities of Δ6 desaturase in diabetic rats were 68% of those of controls (P < 0.05), and increased to 119% of controls after insulin treatment. These results show that insulin restores the fatty acid composition earlier in the liver microsome than in the erythrocyte membrane in STZ-induced diabetic rats. The erythrocyte membrane would not be suitable for the investigation dealing with rapid changes of fatty acid composition.

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KW - Δ6 desaturase

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