In vivo photoacoustic mouse eye imaging of healing after chemical injury and automated eyeball surface estimation based on a random sample consensus algorithm

Seungwan Jeon, Hyun Beom Song, Jaewoo Kim, Byung Joo Lee, Jeong Hun Kim, Chulhong Kim

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Ocular chemical damage may induce limbal vessel ischemia and neovascularization, but the pathophysiology of the disease is not completely known. To observe changes in blood vessels after alkaline burn, we monitored the anterior segment and choroidal vasculature using a photoacoustic microscope (OR-PAM). We were able to observe not only the iris blood vessels but also the choroidal vessels under the sclera, which were difficult to be observed with conventional photographs. After alkali burning, we observed neovascularization and limbal ischemia and successfully tracked changes in vasculature during the 7-day healing process. We also used the RANdom SAmple Consensus (RANSAC) method to segment the abnormally generated blood vessels in the cornea by detecting the eyeball surface and successfully visualize the distance from each PA signal to the center of the eye. We believe that photoacoustic imaging has an important potential to reveal the pathophysiology of limb ischemia and neovascularization.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2017
EditorsAlexander A. Oraevsky, Lihong V. Wang
PublisherSPIE
ISBN (Electronic)9781510605695
DOIs
StatePublished - 2017
EventPhotons Plus Ultrasound: Imaging and Sensing 2017 - San Francisco, United States
Duration: 29 Jan 20171 Feb 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10064
ISSN (Print)1605-7422

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2017
Country/TerritoryUnited States
CitySan Francisco
Period29/01/171/02/17

Keywords

  • Image processing
  • In vivo biomedical imaging
  • Neovascularisation
  • Ophthalmology
  • Optical-resolution PAM
  • Photoacoustic microscopy
  • Random sample consensus
  • Surface fitting

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