In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

Daisuke Sugiyama, Sung Won Hur, Anthony E. Pickering, Daisuke Kase, Sangjeong Kim, Mikito Kawamata, Keiji Imoto, Hidemasa Furue

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20 Citations (Scopus)

Abstract

Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

Original languageEnglish
Pages (from-to)2225-2231
Number of pages7
JournalJournal of Physiology
Volume590
Issue number10
DOIs
StatePublished - 1 May 2012

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Locus Coeruleus
Brain Stem
Neurons
6-Cyano-7-nitroquinoxaline-2,3-dione
Pain
Adrenergic Neurons
Patch-Clamp Techniques
Hindlimb
Arousal
Membrane Potentials
Action Potentials

Cite this

Sugiyama, D., Hur, S. W., Pickering, A. E., Kase, D., Kim, S., Kawamata, M., ... Furue, H. (2012). In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem. Journal of Physiology, 590(10), 2225-2231. https://doi.org/10.1113/jphysiol.2011.226407
Sugiyama, Daisuke ; Hur, Sung Won ; Pickering, Anthony E. ; Kase, Daisuke ; Kim, Sangjeong ; Kawamata, Mikito ; Imoto, Keiji ; Furue, Hidemasa. / In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem. In: Journal of Physiology. 2012 ; Vol. 590, No. 10. pp. 2225-2231.
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Sugiyama, D, Hur, SW, Pickering, AE, Kase, D, Kim, S, Kawamata, M, Imoto, K & Furue, H 2012, 'In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem', Journal of Physiology, vol. 590, no. 10, pp. 2225-2231. https://doi.org/10.1113/jphysiol.2011.226407

In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem. / Sugiyama, Daisuke; Hur, Sung Won; Pickering, Anthony E.; Kase, Daisuke; Kim, Sangjeong; Kawamata, Mikito; Imoto, Keiji; Furue, Hidemasa.

In: Journal of Physiology, Vol. 590, No. 10, 01.05.2012, p. 2225-2231.

Research output: Contribution to journalArticleResearchpeer-review

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