In vitro mammary duct reconstruction based localized collagen extracellular matrix in microfluidic platform

Youngkyu Cho, Su Hyun Lee, Yoojin Shin, Jaehoon Kim, Seok Chung, Wookyung Moon

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Abstract

Reconstruction of mammary duct in vitro is highly required to study breast cancer pathology in progressing invasive ductal carcinoma in situ (DCIS) [1]. 30% of DCIS cases were known to have invasive character, which is strongly associated with cancer malignancy. Understanding the invasive transition needs an in vitro model with modulating capability on microenvironment, consisting of mammary duct, stromal cells and extracellular matrix (ECM). Here, we mimicked the mammary duct in microfluidic platform in 3D on type I collagen ECM. Morphologies and functions of the mammary duct were confirmed by 3D lumen structure, junction proteins and permeability.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages525-526
Number of pages2
ISBN (Electronic)9780979806490
StatePublished - 1 Jan 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period9/10/1613/10/16

Fingerprint

Microfluidics
Collagen
Ducts
Pathology
Proteins

Keywords

  • Mammary duct
  • Microfluidics

Cite this

Cho, Y., Lee, S. H., Shin, Y., Kim, J., Chung, S., & Moon, W. (2016). In vitro mammary duct reconstruction based localized collagen extracellular matrix in microfluidic platform. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 525-526). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Cho, Youngkyu ; Lee, Su Hyun ; Shin, Yoojin ; Kim, Jaehoon ; Chung, Seok ; Moon, Wookyung. / In vitro mammary duct reconstruction based localized collagen extracellular matrix in microfluidic platform. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 525-526 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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abstract = "Reconstruction of mammary duct in vitro is highly required to study breast cancer pathology in progressing invasive ductal carcinoma in situ (DCIS) [1]. 30{\%} of DCIS cases were known to have invasive character, which is strongly associated with cancer malignancy. Understanding the invasive transition needs an in vitro model with modulating capability on microenvironment, consisting of mammary duct, stromal cells and extracellular matrix (ECM). Here, we mimicked the mammary duct in microfluidic platform in 3D on type I collagen ECM. Morphologies and functions of the mammary duct were confirmed by 3D lumen structure, junction proteins and permeability.",
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Cho, Y, Lee, SH, Shin, Y, Kim, J, Chung, S & Moon, W 2016, In vitro mammary duct reconstruction based localized collagen extracellular matrix in microfluidic platform. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 525-526, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 9/10/16.

In vitro mammary duct reconstruction based localized collagen extracellular matrix in microfluidic platform. / Cho, Youngkyu; Lee, Su Hyun; Shin, Yoojin; Kim, Jaehoon; Chung, Seok; Moon, Wookyung.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 525-526 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

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Cho Y, Lee SH, Shin Y, Kim J, Chung S, Moon W. In vitro mammary duct reconstruction based localized collagen extracellular matrix in microfluidic platform. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 525-526. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).