Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates

Sook Hyang Jeong, Ming Qiao, Michelina Nascimbeni, Zongyi Hu, Barbara Rehermann, Krishna Murthy, T. Jake Liang

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Abstract

We have previously reported the production of hepatitis C virus-like particles (HCV-LP) using a recombinant baculovirus containing the cDNA of the HCV structural proteins (core, E1, and E2). These particles resemble the putative HCV virions and are capable of inducing strong and broad humoral and cellular immune responses in mice. Here we present evidence on the immunogenicity of HCV-LP and the effects of novel adjuvant systems in a nonhuman primate model, the baboon. Three groups of four baboons were immunized with HCV-LP, HCV-LP and adjuvant AS01B (monophosphoryl lipid A and QS21), or HCV-LP and the combination of AS01B and CpG oligodeoxynucleotides 10105. After four immunizations over an 8-month period, all animals developed HCV-specific humoral and cellular immune responses including antibodies to HCV structural proteins and gamma interferon+ (IFN-γ+)CD4+ and IFN-γ+CD8+ T-cell responses. The immunogenicity of HCV-LP was only marginally enhanced by the use of adjuvants. The overall HCV-specific immune responses were broad and long lasting. Our results suggest that HCV-LP is a potent immunogen to induce HCV-specific humoral and cellular immune responses in primates and may be a promising approach to develop novel preventive and therapeutic modalities.

Original languageEnglish
Pages (from-to)6995-7003
Number of pages9
JournalJournal of Virology
Volume78
Issue number13
DOIs
StatePublished - 1 Jul 2004

Fingerprint

Hepatitis C virus
virus-like particles
Humoral Immunity
humoral immunity
Cellular Immunity
Hepacivirus
cell-mediated immunity
Virion
Primates
Immunization
immunization
adjuvants
structural proteins
Papio
immune response
Baculoviridae
interferons
Hepatitis C Antibodies
interferon-gamma
Oligodeoxyribonucleotides

Cite this

Jeong, Sook Hyang ; Qiao, Ming ; Nascimbeni, Michelina ; Hu, Zongyi ; Rehermann, Barbara ; Murthy, Krishna ; Liang, T. Jake. / Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. In: Journal of Virology. 2004 ; Vol. 78, No. 13. pp. 6995-7003.
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abstract = "We have previously reported the production of hepatitis C virus-like particles (HCV-LP) using a recombinant baculovirus containing the cDNA of the HCV structural proteins (core, E1, and E2). These particles resemble the putative HCV virions and are capable of inducing strong and broad humoral and cellular immune responses in mice. Here we present evidence on the immunogenicity of HCV-LP and the effects of novel adjuvant systems in a nonhuman primate model, the baboon. Three groups of four baboons were immunized with HCV-LP, HCV-LP and adjuvant AS01B (monophosphoryl lipid A and QS21), or HCV-LP and the combination of AS01B and CpG oligodeoxynucleotides 10105. After four immunizations over an 8-month period, all animals developed HCV-specific humoral and cellular immune responses including antibodies to HCV structural proteins and gamma interferon+ (IFN-γ+)CD4+ and IFN-γ+CD8+ T-cell responses. The immunogenicity of HCV-LP was only marginally enhanced by the use of adjuvants. The overall HCV-specific immune responses were broad and long lasting. Our results suggest that HCV-LP is a potent immunogen to induce HCV-specific humoral and cellular immune responses in primates and may be a promising approach to develop novel preventive and therapeutic modalities.",
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Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. / Jeong, Sook Hyang; Qiao, Ming; Nascimbeni, Michelina; Hu, Zongyi; Rehermann, Barbara; Murthy, Krishna; Liang, T. Jake.

In: Journal of Virology, Vol. 78, No. 13, 01.07.2004, p. 6995-7003.

Research output: Contribution to journalArticleResearchpeer-review

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