IL-2/anti-IL-2 complex attenuates renal ischemia-reperfusion injury through expansion of regulatory T cells

Myung Gyu Kim, Tai Yeon Koo, Ji Jing Yan, Eunwon Lee, Kyu Hyun Han, Jong Cheol Jeong, Han Ro, Beom Seok Kim, Sang Kyung Jo, Kook Hwan Oh, Charles D. Surh, Curie Ahn, Jaeseok Yang

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Abstract

Regulatory T cells (Tregs) can suppress immunologic damage in renal ischemiareperfusion injury (IRI), but the isolation and ex vivo expansion of these cells for clinical application remains challenging. Here, we investigated whether the IL-2/anti-IL-2 complex (IL-2C), a mediator of Treg expansion, can attenuate renal IRI in mice. IL-2C administered before bilateral renal IRI induced Treg expansion in both spleen and kidney, improved renal function, and attenuated histologic renal injury and apoptosis after IRI. Furthermore, IL-2C administration reduced the expression of inflammatory cytokines and attenuated the infiltration of neutrophils and macrophages in renal tissue. Depletion of Tregs with anti-CD25 antibodies abrogated the beneficial effects of IL-2C. However, IL-2C-mediated renal protection was not dependent on either IL-10or TGF-b. Notably, IL-2Cadministered after IRI also enhanced Treg expansion in spleen and kidney, increased tubular cell proliferation, improved renal function, and reduced renal fibrosis. In conclusion, these results indicate that IL-2C-induced Tregexpansion attenuates acute renal damage and improves renal recovery in vivo, suggesting that IL-2C may be a therapeutic strategy for renal IRI.

Original languageEnglish
Pages (from-to)1529-1536
Number of pages8
JournalJournal of the American Society of Nephrology
Volume24
Issue number10
DOIs
StatePublished - 1 Oct 2013

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