IFN-γ-induced BST2 mediates monocyte adhesion to human endothelial cells

Hyouna Yoo, Sang Ho Park, Sang Kyu Ye, Myung Kim

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

BST2 is a type II transmembrane protein that had been initially identified as a surface molecule expressed on terminally differentiated B cells. Here, we characterize the expression of BST2 in human endothelial cells, HUVECs. IFN-γ, among various inflammatory stimuli, dramatically upregulates BST2 expression in HUVECs. We also address a novel putative role of BST2 in IFN-γ-stimulated HUVECs as an intercellular adhesion-related molecule. We show that purified extracellular domain of BST2 protein specifically and significantly decreased the adhesion of human monocytes to HUVECs, which suggests that IFN-γ-induced BST2 expression may be involved in monocyte migration from blood through the endothelium to the inflammation site. Furthermore, we show that the monocytic cell line U937 can directly adhere to BST2 extracellular domain-coated tissue culture wells. These results provide experimental evidence to support a novel role for BST2 in the interaction between human monocyte and IFN-γ-stimulated endothelium.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalCellular Immunology
Volume267
Issue number1
DOIs
StatePublished - 1 Jan 2011

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Monocytes
Endothelial Cells
Endothelium
Cell Adhesion Molecules
B-Lymphocytes
Up-Regulation
Inflammation
Cell Line
Proteins
Protein Domains

Keywords

  • BST2
  • Endothelial cell adhesion
  • Interferon gamma (IFN-γ)
  • Monocyte

Cite this

Yoo, Hyouna ; Park, Sang Ho ; Ye, Sang Kyu ; Kim, Myung. / IFN-γ-induced BST2 mediates monocyte adhesion to human endothelial cells. In: Cellular Immunology. 2011 ; Vol. 267, No. 1. pp. 23-29.
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IFN-γ-induced BST2 mediates monocyte adhesion to human endothelial cells. / Yoo, Hyouna; Park, Sang Ho; Ye, Sang Kyu; Kim, Myung.

In: Cellular Immunology, Vol. 267, No. 1, 01.01.2011, p. 23-29.

Research output: Contribution to journalArticle

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