Identification of nuclear/nucleolar localization signal in Aplysia learning associated protein of slug with a molecular mass of 18 kDa homologous protein

Hyoung Kim, Deok Jin Chang, Jin A. Lee, Yong-Seok Lee, Bong Kiun Kaang

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

We isolated a learning associated protein of slug with a molecular mass of 18 kDa (LAPS18) homologue from the expressed sequence tag database of Aplysia kurodai and named it Aplysia LAPS18-like protein (ApLLP). ApLLP encodes 120 amino acids and has 57% identity with LAPS18. To examine the subcellular expression pattern of ApLLP we constructed an EGFP-tagged ApLLP fusion protein and overexpressed it in both Aplysia neurons and COS-7 cells. In contrast to the previous findings, which showed that LAPS18 is secreted by COS-7 cells, ApLLP-EGFP was localized to the nucleus, and most of it to nucleoli. Analysis of deletion mutants of ApLLP-EGFP showed that the N-terminal and the C-terminal nucleolar and nucleus localization signal sequences are important for localization to the nucleus and the nucleoli.

Original languageEnglish
Pages (from-to)134-138
Number of pages5
JournalNeuroscience Letters
Volume343
Issue number2
DOIs
StatePublished - 5 Jun 2003

Fingerprint

Aplysia
Nuclear Localization Signals
Gastropoda
Learning
Proteins
COS Cells
Expressed Sequence Tags
Protein Sorting Signals
Databases
Neurons
Amino Acids

Keywords

  • LAPS18
  • Nuclear localization signal sequence
  • Nucleolar localization signal sequence
  • Nucleolus
  • Nucleus

Cite this

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title = "Identification of nuclear/nucleolar localization signal in Aplysia learning associated protein of slug with a molecular mass of 18 kDa homologous protein",
abstract = "We isolated a learning associated protein of slug with a molecular mass of 18 kDa (LAPS18) homologue from the expressed sequence tag database of Aplysia kurodai and named it Aplysia LAPS18-like protein (ApLLP). ApLLP encodes 120 amino acids and has 57{\%} identity with LAPS18. To examine the subcellular expression pattern of ApLLP we constructed an EGFP-tagged ApLLP fusion protein and overexpressed it in both Aplysia neurons and COS-7 cells. In contrast to the previous findings, which showed that LAPS18 is secreted by COS-7 cells, ApLLP-EGFP was localized to the nucleus, and most of it to nucleoli. Analysis of deletion mutants of ApLLP-EGFP showed that the N-terminal and the C-terminal nucleolar and nucleus localization signal sequences are important for localization to the nucleus and the nucleoli.",
keywords = "LAPS18, Nuclear localization signal sequence, Nucleolar localization signal sequence, Nucleolus, Nucleus",
author = "Hyoung Kim and Chang, {Deok Jin} and Lee, {Jin A.} and Yong-Seok Lee and Kaang, {Bong Kiun}",
year = "2003",
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language = "English",
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Identification of nuclear/nucleolar localization signal in Aplysia learning associated protein of slug with a molecular mass of 18 kDa homologous protein. / Kim, Hyoung; Chang, Deok Jin; Lee, Jin A.; Lee, Yong-Seok; Kaang, Bong Kiun.

In: Neuroscience Letters, Vol. 343, No. 2, 05.06.2003, p. 134-138.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Identification of nuclear/nucleolar localization signal in Aplysia learning associated protein of slug with a molecular mass of 18 kDa homologous protein

AU - Kim, Hyoung

AU - Chang, Deok Jin

AU - Lee, Jin A.

AU - Lee, Yong-Seok

AU - Kaang, Bong Kiun

PY - 2003/6/5

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AB - We isolated a learning associated protein of slug with a molecular mass of 18 kDa (LAPS18) homologue from the expressed sequence tag database of Aplysia kurodai and named it Aplysia LAPS18-like protein (ApLLP). ApLLP encodes 120 amino acids and has 57% identity with LAPS18. To examine the subcellular expression pattern of ApLLP we constructed an EGFP-tagged ApLLP fusion protein and overexpressed it in both Aplysia neurons and COS-7 cells. In contrast to the previous findings, which showed that LAPS18 is secreted by COS-7 cells, ApLLP-EGFP was localized to the nucleus, and most of it to nucleoli. Analysis of deletion mutants of ApLLP-EGFP showed that the N-terminal and the C-terminal nucleolar and nucleus localization signal sequences are important for localization to the nucleus and the nucleoli.

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KW - Nucleolar localization signal sequence

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