Identification of microRNAs modulated by DNA hypomethylating drugs in extranodal NK/T-cell lymphoma

Heounjeong Go, Ji Young Jang, Chul Woo Kim, Jooryung Huh, Pil Jong Kim, Yoon Kyung Jeon

Research output: Contribution to journalArticle

Abstract

To identify epigenetically silenced miRNAs and to investigate their influences on predictive target oncogenes in extranodal natural killer/T-cell lymphoma (NKTCL). Decitabine treatment was performed to evaluate methylated miRNAs in NKTCL cells. The relationship between a given miRNA and its target mRNA was validated using 24 tumor tissues. miR-379, miR-134, miR-20b, miR-376a, miR-654-3p, miR-143, miR-181c, miR-1225-5p, miR-1246, and miR-1275 were epigenetically silenced in SNK6 cells. miR-134, miR-376a, miR-143 and miR-181c significantly affected cellular viability. PDGFRα was regulated by miR-34a and miR-181c. miR-143, miR-20b and miR34a regulated STAT3 expression. miR-20b and miR-143 expression showed inverse correlations with STAT3 mRNA expression in NKTCL tissues. K-RAS was regulated by miR-181c. Downregulation of cell viability by salirasib treatment was identified. miRNAs were downregulated by DNA methylation, and several microRNAs affected the viability of NKTCL cells. miR-34a and miR-181c may be involved in the oncogenic progression of NKTCL through the regulation of PDGFRα, STAT3, and K-RAS.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalLeukemia and Lymphoma
Volume61
Issue number1
DOIs
StatePublished - 2 Jan 2020

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Extranodal NK-T-Cell Lymphoma
Natural Killer T-Cells
T-Cell Lymphoma
MicroRNAs
DNA
Pharmaceutical Preparations
decitabine
Down-Regulation
Messenger RNA
DNA Methylation
Oncogenes
Cell Survival
Neoplasms

Keywords

  • DNA methylation
  • extranodal natural killer/T-cell lymphoma
  • miR-181c
  • miR-34a
  • miRNA

Cite this

Go, Heounjeong ; Jang, Ji Young ; Kim, Chul Woo ; Huh, Jooryung ; Kim, Pil Jong ; Jeon, Yoon Kyung. / Identification of microRNAs modulated by DNA hypomethylating drugs in extranodal NK/T-cell lymphoma. In: Leukemia and Lymphoma. 2020 ; Vol. 61, No. 1. pp. 66-74.
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Identification of microRNAs modulated by DNA hypomethylating drugs in extranodal NK/T-cell lymphoma. / Go, Heounjeong; Jang, Ji Young; Kim, Chul Woo; Huh, Jooryung; Kim, Pil Jong; Jeon, Yoon Kyung.

In: Leukemia and Lymphoma, Vol. 61, No. 1, 02.01.2020, p. 66-74.

Research output: Contribution to journalArticle

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AU - Go, Heounjeong

AU - Jang, Ji Young

AU - Kim, Chul Woo

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AU - Kim, Pil Jong

AU - Jeon, Yoon Kyung

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