Identification of genes differentially expressed by hypoxia in hepatocellular carcinoma cells

Moon Kyoung Bae, Yoo Wook Kwon, Myoung Sook Kim, Soo Kyung Bae, Myung Ho Bae, You Mie Lee, Yung Jin Kim, Kyu Won Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In order to identify genes differentially expressed under hypoxia (1% O2, 5% CO2, balance N2), we performed mRNA differential display analysis using total RNA extracted from hypoxic and normoxic HepG2, human hepatocellular carcinoma (HCC) cells. Of the differentially expressed genes by hypoxia, some of cDNA fragments were cloned and sequenced. The expression patterns of these clones by hypoxia were confirmed by Northern blot analysis and the quantitative RT-PCR. Down-regulated genes by hypoxia have homology to cDNA sequences encoding cytochrome oxidase subunit II and ADP/ATP translocase, respectively. Up-regulated gene by hypoxia was identified as Homo sapiens oscillin. Moreover, novel genes induced by hypoxia represent partial sequences of cDNAs that have not been reported or functionally identified. Up- or down-regulated expression of these genes in response to hypoxia may contribute to human hepatocarcinogenesis.

Original languageEnglish
Pages (from-to)158-162
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume243
Issue number1
DOIs
StatePublished - 4 Feb 1998

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Hepatocellular Carcinoma
Genes
Cells
Complementary DNA
ATP Translocases Mitochondrial ADP
Electron Transport Complex IV
Gene Expression Profiling
Hypoxia
Northern Blotting
Display devices
RNA
Clone Cells
Messenger RNA
Gene Expression
Polymerase Chain Reaction

Cite this

Bae, Moon Kyoung ; Kwon, Yoo Wook ; Kim, Myoung Sook ; Bae, Soo Kyung ; Bae, Myung Ho ; Lee, You Mie ; Kim, Yung Jin ; Kim, Kyu Won. / Identification of genes differentially expressed by hypoxia in hepatocellular carcinoma cells. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 243, No. 1. pp. 158-162.
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Identification of genes differentially expressed by hypoxia in hepatocellular carcinoma cells. / Bae, Moon Kyoung; Kwon, Yoo Wook; Kim, Myoung Sook; Bae, Soo Kyung; Bae, Myung Ho; Lee, You Mie; Kim, Yung Jin; Kim, Kyu Won.

In: Biochemical and Biophysical Research Communications, Vol. 243, No. 1, 04.02.1998, p. 158-162.

Research output: Contribution to journalArticle

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