Identification of fastidious mycobacteria other than M. tuberculosis (MOTT) by comparative sequence analysis of rpoB and 16S rDNA

Bum Joon Kim, Seong In Im, Youn Woo Kim, Gill Han Bai, Sang Jae Kim, Keun Hwa Lee, Chang Yong Cha, Yoon Hoh Kook

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Abstract

Conventional tests for the identification of mycobacteria may frequently result in erroneous identification and underestimate the diversity within the genus Mycobacterium. However, this problem can be overcome by molecular approach like as 16S rRNA gene (rDNA) or RNA polymerase gene (rpoB) sequence analysis. In this study, a molecular approach analyzing partial sequence of 16S rDNA and rpoB gene was applied to mycobacteria other than M. tuberculosis (MOTT) isolates that had not been definitely identified by conventional physical and biochemical tests. Among the eighteen isolates included in this study, twelve isolates could be identified to the species level and six were identified to the complex level. Compared with the results by 16S rDNA analysis, the rpoB analysis could differentiate some of the strains into the subspecies level.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalJournal of Bacteriology and Virology
Volume32
Issue number1
StatePublished - 1 Jan 2002

Keywords

  • 16S rDNA
  • Identification
  • Mycobacteria other than M. Tuberculosis (MOTT)
  • rpoB

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