Hypertensive left ventricular hypertrophy

Relation to β-adrenergic receptor kinase-1 (βARK1) in peripheral lymphocytes

Sung Ji Park, Dong-Ju Choi, Choong Won Kim

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Background: Left ventricular hypertrophy (LVH) is associated with increased cardiovascular risk and altered sympathetic regulation in hypertension. Objectives: To determine whether the level of β-adrenergic receptor kinase-1 (βARK1) in lymphocytes is related to LVH in patients with hypertension. Methods: Forty-nine patients with untreated essential hypertension were recruited to the study and classified into two groups: left ventricular hypertrophy (LVH: left ventricular mass index ≥ 134 g/m 2 in men and ≥ 110 g/ m 2 in women; ages 52.4 ± 12.8 years, n = 25) and non-LVH (NLVH: left ventricular mass index < 134 g/m 2 in men and < 110 g/m 2 in women; ages 50.8 ± 13.1 years, n = 24). Lymphocytes were isolated from patients and quantitative-competitive reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to estimate the expression of βARK1 in the lymphocytes. G-protein-coupled receptor kinase activity was assessed by rhodopsin phosphorylation assay. Results: The expression of βARK1 in lymphocytes was greater in the LVH group than in the NLVH group (0.0069 ± 0.002 ng compared with 0.0048 ± 0.0017 ng, P < 0.01) and correlated well with left ventricular mass index (r = 0.527, P < 0.001) and relative wall thickness (r = 0.627, P < 0.001). The concentration of βARK1 protein in lymphocytes from individuals with LVH was increased two-fold compared with that in the NLVM group (both n = 7). Lymphocyte G-protein-coupled receptor kinase activity from LVH was enhanced 1.7-fold compared with NLVH (1.03 ± 2.16 and 1.79 ± 1.87 pmol phosphate/min per mg protein, respectively; P < 0.05, n = 7 for each group). Conclusions: The concentration of βARK1 in lymphocytes is greater in hypertensive individuals with LVH than in those without LVH and parallels the degree of hypertrophy. Generalized alterations in β-adrenergic signalling, including βARK1, could be a major contributory factor in the development of LVH in hypertension, and the concentration of βARK1 in lymphocytes can reflect the development of LVH in a patient with hypertension.

Original languageEnglish
Pages (from-to)1025-1032
Number of pages8
JournalJournal of Hypertension
Volume22
Issue number5
DOIs
StatePublished - 1 May 2004

Fingerprint

Left Ventricular Hypertrophy
Adrenergic Receptors
Phosphotransferases
Lymphocytes
G-Protein-Coupled Receptor Kinases
Hypertension
Rhodopsin
Reverse Transcriptase Polymerase Chain Reaction
Immunoblotting
Adrenergic Agents
Protein Kinases
Hypertrophy
Phosphates
Phosphorylation

Keywords

  • Cardiac hypertrophy
  • Hypertension
  • Lymphocyte
  • β-adrenergic receptor kinase-1

Cite this

@article{a57e0936636541c1a39f34e74cf9bebd,
title = "Hypertensive left ventricular hypertrophy: Relation to β-adrenergic receptor kinase-1 (βARK1) in peripheral lymphocytes",
abstract = "Background: Left ventricular hypertrophy (LVH) is associated with increased cardiovascular risk and altered sympathetic regulation in hypertension. Objectives: To determine whether the level of β-adrenergic receptor kinase-1 (βARK1) in lymphocytes is related to LVH in patients with hypertension. Methods: Forty-nine patients with untreated essential hypertension were recruited to the study and classified into two groups: left ventricular hypertrophy (LVH: left ventricular mass index ≥ 134 g/m 2 in men and ≥ 110 g/ m 2 in women; ages 52.4 ± 12.8 years, n = 25) and non-LVH (NLVH: left ventricular mass index < 134 g/m 2 in men and < 110 g/m 2 in women; ages 50.8 ± 13.1 years, n = 24). Lymphocytes were isolated from patients and quantitative-competitive reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to estimate the expression of βARK1 in the lymphocytes. G-protein-coupled receptor kinase activity was assessed by rhodopsin phosphorylation assay. Results: The expression of βARK1 in lymphocytes was greater in the LVH group than in the NLVH group (0.0069 ± 0.002 ng compared with 0.0048 ± 0.0017 ng, P < 0.01) and correlated well with left ventricular mass index (r = 0.527, P < 0.001) and relative wall thickness (r = 0.627, P < 0.001). The concentration of βARK1 protein in lymphocytes from individuals with LVH was increased two-fold compared with that in the NLVM group (both n = 7). Lymphocyte G-protein-coupled receptor kinase activity from LVH was enhanced 1.7-fold compared with NLVH (1.03 ± 2.16 and 1.79 ± 1.87 pmol phosphate/min per mg protein, respectively; P < 0.05, n = 7 for each group). Conclusions: The concentration of βARK1 in lymphocytes is greater in hypertensive individuals with LVH than in those without LVH and parallels the degree of hypertrophy. Generalized alterations in β-adrenergic signalling, including βARK1, could be a major contributory factor in the development of LVH in hypertension, and the concentration of βARK1 in lymphocytes can reflect the development of LVH in a patient with hypertension.",
keywords = "Cardiac hypertrophy, Hypertension, Lymphocyte, β-adrenergic receptor kinase-1",
author = "Park, {Sung Ji} and Dong-Ju Choi and Kim, {Choong Won}",
year = "2004",
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language = "English",
volume = "22",
pages = "1025--1032",
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}

Hypertensive left ventricular hypertrophy : Relation to β-adrenergic receptor kinase-1 (βARK1) in peripheral lymphocytes. / Park, Sung Ji; Choi, Dong-Ju; Kim, Choong Won.

In: Journal of Hypertension, Vol. 22, No. 5, 01.05.2004, p. 1025-1032.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Hypertensive left ventricular hypertrophy

T2 - Relation to β-adrenergic receptor kinase-1 (βARK1) in peripheral lymphocytes

AU - Park, Sung Ji

AU - Choi, Dong-Ju

AU - Kim, Choong Won

PY - 2004/5/1

Y1 - 2004/5/1

N2 - Background: Left ventricular hypertrophy (LVH) is associated with increased cardiovascular risk and altered sympathetic regulation in hypertension. Objectives: To determine whether the level of β-adrenergic receptor kinase-1 (βARK1) in lymphocytes is related to LVH in patients with hypertension. Methods: Forty-nine patients with untreated essential hypertension were recruited to the study and classified into two groups: left ventricular hypertrophy (LVH: left ventricular mass index ≥ 134 g/m 2 in men and ≥ 110 g/ m 2 in women; ages 52.4 ± 12.8 years, n = 25) and non-LVH (NLVH: left ventricular mass index < 134 g/m 2 in men and < 110 g/m 2 in women; ages 50.8 ± 13.1 years, n = 24). Lymphocytes were isolated from patients and quantitative-competitive reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to estimate the expression of βARK1 in the lymphocytes. G-protein-coupled receptor kinase activity was assessed by rhodopsin phosphorylation assay. Results: The expression of βARK1 in lymphocytes was greater in the LVH group than in the NLVH group (0.0069 ± 0.002 ng compared with 0.0048 ± 0.0017 ng, P < 0.01) and correlated well with left ventricular mass index (r = 0.527, P < 0.001) and relative wall thickness (r = 0.627, P < 0.001). The concentration of βARK1 protein in lymphocytes from individuals with LVH was increased two-fold compared with that in the NLVM group (both n = 7). Lymphocyte G-protein-coupled receptor kinase activity from LVH was enhanced 1.7-fold compared with NLVH (1.03 ± 2.16 and 1.79 ± 1.87 pmol phosphate/min per mg protein, respectively; P < 0.05, n = 7 for each group). Conclusions: The concentration of βARK1 in lymphocytes is greater in hypertensive individuals with LVH than in those without LVH and parallels the degree of hypertrophy. Generalized alterations in β-adrenergic signalling, including βARK1, could be a major contributory factor in the development of LVH in hypertension, and the concentration of βARK1 in lymphocytes can reflect the development of LVH in a patient with hypertension.

AB - Background: Left ventricular hypertrophy (LVH) is associated with increased cardiovascular risk and altered sympathetic regulation in hypertension. Objectives: To determine whether the level of β-adrenergic receptor kinase-1 (βARK1) in lymphocytes is related to LVH in patients with hypertension. Methods: Forty-nine patients with untreated essential hypertension were recruited to the study and classified into two groups: left ventricular hypertrophy (LVH: left ventricular mass index ≥ 134 g/m 2 in men and ≥ 110 g/ m 2 in women; ages 52.4 ± 12.8 years, n = 25) and non-LVH (NLVH: left ventricular mass index < 134 g/m 2 in men and < 110 g/m 2 in women; ages 50.8 ± 13.1 years, n = 24). Lymphocytes were isolated from patients and quantitative-competitive reverse transcriptase-polymerase chain reaction (RT-PCR) and immunoblotting were used to estimate the expression of βARK1 in the lymphocytes. G-protein-coupled receptor kinase activity was assessed by rhodopsin phosphorylation assay. Results: The expression of βARK1 in lymphocytes was greater in the LVH group than in the NLVH group (0.0069 ± 0.002 ng compared with 0.0048 ± 0.0017 ng, P < 0.01) and correlated well with left ventricular mass index (r = 0.527, P < 0.001) and relative wall thickness (r = 0.627, P < 0.001). The concentration of βARK1 protein in lymphocytes from individuals with LVH was increased two-fold compared with that in the NLVM group (both n = 7). Lymphocyte G-protein-coupled receptor kinase activity from LVH was enhanced 1.7-fold compared with NLVH (1.03 ± 2.16 and 1.79 ± 1.87 pmol phosphate/min per mg protein, respectively; P < 0.05, n = 7 for each group). Conclusions: The concentration of βARK1 in lymphocytes is greater in hypertensive individuals with LVH than in those without LVH and parallels the degree of hypertrophy. Generalized alterations in β-adrenergic signalling, including βARK1, could be a major contributory factor in the development of LVH in hypertension, and the concentration of βARK1 in lymphocytes can reflect the development of LVH in a patient with hypertension.

KW - Cardiac hypertrophy

KW - Hypertension

KW - Lymphocyte

KW - β-adrenergic receptor kinase-1

UR - http://www.scopus.com/inward/record.url?scp=2542495281&partnerID=8YFLogxK

U2 - 10.1097/00004872-200405000-00026

DO - 10.1097/00004872-200405000-00026

M3 - Article

VL - 22

SP - 1025

EP - 1032

JO - Journal of Hypertension

JF - Journal of Hypertension

SN - 0263-6352

IS - 5

ER -