Histological findings of regeneration in retrieved pasteurized bone grafts

Won Jong Bahk, Hyun Guy Kang, Seog Yun Park, Hwan-Seong Cho, Yongsin Cho, Kyung Jin Seo

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Despite a number of radiologic evaluations of the incorporation of pasteurized bone (PB) in human and histologic evaluations in animal models, there has been a scarce documentation regarding the histologic evaluation of PB from human. Herein, we present histologic findings of regeneration in retrieved PB graft from pediatric and adult patients. Methods: PB was retrieved for various reasons in 7 patients (10–52 years old). Two bone pathologists independently counted the number of empty lacunae and lacunae with living cells in up to 10 randomly selected fields on medium-power (H&E, ×200) for each patient. Regeneration of PB was assessed as the ratio of the number of lacunae with nucleated cells to that of whole lacunae, which was defined as the “repair rate (RR)”. Results: The mean interval between initial reconstruction and retrieval (graft removal time; GRT) was 47.4 months (range, 11–144 months). The length of original PBs ranged from 5.8 to 20.6 cm. Microscopic examination of PBs showed areas with empty lacunae indicating necrosis and other areas contained lacunae with nucleated osteocytes, indicative of regeneration. Some Haversian canals of the PBs were filled with fibrovascular tissue and surrounded by lamellar bones including living osteocytes. RR varied widely from 21.7 to 62.4% with a mean of 36.8%. It was much higher in adult patients (46.6–62.4%, mean = 55.3%) than in pediatric patients (21.7–28.6%, mean = 25.3%), which was correlated with GRT (pediatric patients; mean of 14 months, adult patients; mean of 72.3 months). In adult patients, RR was higher in a patient with prosthesis composite in the proximal humerus (Case No. 3; 62.4%). Conclusions: RR was higher in whom GRT was longer, being correlated with GRT in retrieved PBs. In terms of our histological observation, PB is thought to be an acceptable temporary biologic spacer in limb-sparing surgery for malignant bone or soft tissue tumors.

Original languageEnglish
Pages (from-to)737-741
Number of pages5
JournalJournal of Orthopaedic Science
Volume24
Issue number4
DOIs
StatePublished - 1 Jul 2019

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Regeneration
Transplants
Bone and Bones
Osteocytes
Pediatrics
Haversian System
Bone Regeneration
Humerus
Documentation
Prostheses and Implants
Necrosis
Extremities
Animal Models
Observation
Neoplasms

Cite this

Bahk, Won Jong ; Kang, Hyun Guy ; Park, Seog Yun ; Cho, Hwan-Seong ; Cho, Yongsin ; Seo, Kyung Jin. / Histological findings of regeneration in retrieved pasteurized bone grafts. In: Journal of Orthopaedic Science. 2019 ; Vol. 24, No. 4. pp. 737-741.
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title = "Histological findings of regeneration in retrieved pasteurized bone grafts",
abstract = "Background: Despite a number of radiologic evaluations of the incorporation of pasteurized bone (PB) in human and histologic evaluations in animal models, there has been a scarce documentation regarding the histologic evaluation of PB from human. Herein, we present histologic findings of regeneration in retrieved PB graft from pediatric and adult patients. Methods: PB was retrieved for various reasons in 7 patients (10–52 years old). Two bone pathologists independently counted the number of empty lacunae and lacunae with living cells in up to 10 randomly selected fields on medium-power (H&E, ×200) for each patient. Regeneration of PB was assessed as the ratio of the number of lacunae with nucleated cells to that of whole lacunae, which was defined as the “repair rate (RR)”. Results: The mean interval between initial reconstruction and retrieval (graft removal time; GRT) was 47.4 months (range, 11–144 months). The length of original PBs ranged from 5.8 to 20.6 cm. Microscopic examination of PBs showed areas with empty lacunae indicating necrosis and other areas contained lacunae with nucleated osteocytes, indicative of regeneration. Some Haversian canals of the PBs were filled with fibrovascular tissue and surrounded by lamellar bones including living osteocytes. RR varied widely from 21.7 to 62.4{\%} with a mean of 36.8{\%}. It was much higher in adult patients (46.6–62.4{\%}, mean = 55.3{\%}) than in pediatric patients (21.7–28.6{\%}, mean = 25.3{\%}), which was correlated with GRT (pediatric patients; mean of 14 months, adult patients; mean of 72.3 months). In adult patients, RR was higher in a patient with prosthesis composite in the proximal humerus (Case No. 3; 62.4{\%}). Conclusions: RR was higher in whom GRT was longer, being correlated with GRT in retrieved PBs. In terms of our histological observation, PB is thought to be an acceptable temporary biologic spacer in limb-sparing surgery for malignant bone or soft tissue tumors.",
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Histological findings of regeneration in retrieved pasteurized bone grafts. / Bahk, Won Jong; Kang, Hyun Guy; Park, Seog Yun; Cho, Hwan-Seong; Cho, Yongsin; Seo, Kyung Jin.

In: Journal of Orthopaedic Science, Vol. 24, No. 4, 01.07.2019, p. 737-741.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Histological findings of regeneration in retrieved pasteurized bone grafts

AU - Bahk, Won Jong

AU - Kang, Hyun Guy

AU - Park, Seog Yun

AU - Cho, Hwan-Seong

AU - Cho, Yongsin

AU - Seo, Kyung Jin

PY - 2019/7/1

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N2 - Background: Despite a number of radiologic evaluations of the incorporation of pasteurized bone (PB) in human and histologic evaluations in animal models, there has been a scarce documentation regarding the histologic evaluation of PB from human. Herein, we present histologic findings of regeneration in retrieved PB graft from pediatric and adult patients. Methods: PB was retrieved for various reasons in 7 patients (10–52 years old). Two bone pathologists independently counted the number of empty lacunae and lacunae with living cells in up to 10 randomly selected fields on medium-power (H&E, ×200) for each patient. Regeneration of PB was assessed as the ratio of the number of lacunae with nucleated cells to that of whole lacunae, which was defined as the “repair rate (RR)”. Results: The mean interval between initial reconstruction and retrieval (graft removal time; GRT) was 47.4 months (range, 11–144 months). The length of original PBs ranged from 5.8 to 20.6 cm. Microscopic examination of PBs showed areas with empty lacunae indicating necrosis and other areas contained lacunae with nucleated osteocytes, indicative of regeneration. Some Haversian canals of the PBs were filled with fibrovascular tissue and surrounded by lamellar bones including living osteocytes. RR varied widely from 21.7 to 62.4% with a mean of 36.8%. It was much higher in adult patients (46.6–62.4%, mean = 55.3%) than in pediatric patients (21.7–28.6%, mean = 25.3%), which was correlated with GRT (pediatric patients; mean of 14 months, adult patients; mean of 72.3 months). In adult patients, RR was higher in a patient with prosthesis composite in the proximal humerus (Case No. 3; 62.4%). Conclusions: RR was higher in whom GRT was longer, being correlated with GRT in retrieved PBs. In terms of our histological observation, PB is thought to be an acceptable temporary biologic spacer in limb-sparing surgery for malignant bone or soft tissue tumors.

AB - Background: Despite a number of radiologic evaluations of the incorporation of pasteurized bone (PB) in human and histologic evaluations in animal models, there has been a scarce documentation regarding the histologic evaluation of PB from human. Herein, we present histologic findings of regeneration in retrieved PB graft from pediatric and adult patients. Methods: PB was retrieved for various reasons in 7 patients (10–52 years old). Two bone pathologists independently counted the number of empty lacunae and lacunae with living cells in up to 10 randomly selected fields on medium-power (H&E, ×200) for each patient. Regeneration of PB was assessed as the ratio of the number of lacunae with nucleated cells to that of whole lacunae, which was defined as the “repair rate (RR)”. Results: The mean interval between initial reconstruction and retrieval (graft removal time; GRT) was 47.4 months (range, 11–144 months). The length of original PBs ranged from 5.8 to 20.6 cm. Microscopic examination of PBs showed areas with empty lacunae indicating necrosis and other areas contained lacunae with nucleated osteocytes, indicative of regeneration. Some Haversian canals of the PBs were filled with fibrovascular tissue and surrounded by lamellar bones including living osteocytes. RR varied widely from 21.7 to 62.4% with a mean of 36.8%. It was much higher in adult patients (46.6–62.4%, mean = 55.3%) than in pediatric patients (21.7–28.6%, mean = 25.3%), which was correlated with GRT (pediatric patients; mean of 14 months, adult patients; mean of 72.3 months). In adult patients, RR was higher in a patient with prosthesis composite in the proximal humerus (Case No. 3; 62.4%). Conclusions: RR was higher in whom GRT was longer, being correlated with GRT in retrieved PBs. In terms of our histological observation, PB is thought to be an acceptable temporary biologic spacer in limb-sparing surgery for malignant bone or soft tissue tumors.

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