Histologic characteristics and mechanical properties of bovine pericardium treated with decellularization and α-galactosidase: Acomparative study

Byoung Ju Min, Yong Jin Kim, Jae Woong Choi, Sun Young Choi, Soo Hwan Kim, Hong Gook Lim

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Background: Bioprostheses for cardiovascular surgery have limitations in their use following as calicification. α-galactosidase epitope is known as a stimulant of immune response and then shows a progressing calcification. The objective of this study was to evaluate histologic characteristics and mechanical properties of decellularization and treated with α-galactosidase. Materials and Methods: Bovine pericardial tissues were allocated into three groups: fixation only with glutaraldehyde, decellularization with sodium dodesyl sulfate and decellularization plus treatment with α-galactosidase. We confirmed immunohistological characteristics and mechanical properties as fatigue test, permeability test, compliance test, tensile strength (strain) test and thermal stability test. Results: Decellularization and elimination of α-gal were confirmed through immunohistologic findings. Decellularization had decreasedmechanical properties compared to fixation only group in permeability (before fatigue test p=0.02, after fatigue test p=0.034), compliance (after fatigue test p=0.041), and tensile strength test (p=0.00). The group of decellularization plus treatment with α-galactosidase had less desirable mechanical properties than the group of decellularization in concerns of permeability (before fatigue test p=0.043) and strain test (p=0.001). Conclusion: Favorable decellularization and elimination of α-gal were obtained in this study through immunohistologic findings. However, those treatment including decellularization and elimination of α-gal implied the decreased mechanical properties in specific ways. We need more study to complete appropriate bioprosthesis with decellularization and elimination of α-gal including favorable mechanical properties too.

Original languageEnglish
Pages (from-to)368-379
Number of pages12
JournalKorean Journal of Thoracic and Cardiovascular Surgery
Issue number6
StatePublished - 1 Dec 2012


  • Bioprosthesis
  • Decellularization
  • Glutaraldehyde
  • α-Gal

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