HIF1α in tumorigenesis of adenoid cystic carcinoma

Yun Sung Lim, Wonjae Cha, Min Woo Park, Woo Jin Jeong, Soon-Hyun Ahn

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Tumor hypoxia induces hypoxiainducible factor-1α (HIF1α), which can influence tumorigenesis and metastasis. We evaluated the expression of HIF1α and the effect of HIF1α inhibitors in adenoid cystic carcinoma (ACC). Materials and Methods: HIF1α expression was demonstrated in ACC cell lines (ACC2 and ACCM). The effect of HIF1α inhibitors was evaluated. A systemic metastasis model was developed. The number of metastatic pulmonary nodules were analyzed. Results: The ACCM cell line demonstrated greater HIF1α expression and invasion than ACC2. The expression of HIF1α and invasion of ACC cells were blocked by HIF1α siRNA. HIF1α inhibitors 17-Nallylamino-17-demethoxygeldanamycin (17AAG) and echinomycin inhibited cell invasion. 17AAG inhibited metastasis in the animal model, although not statistically significantly. Conclusion: HIF1α siRNA and 17AAG and echinomycin blocked invasion by ACC2 and ACCM cells. 17AAG exhibited therapeutic potential for inhibition of metastasis. Our results provide positive evidence that HIF1α is a promising research pathway for therapy of ACC.

Original languageEnglish
Pages (from-to)599-606
Number of pages8
JournalAnticancer Research
Volume37
Issue number2
DOIs
StatePublished - 1 Jan 2017

Fingerprint

tanespimycin
Adenoid Cystic Carcinoma
Echinomycin
Carcinogenesis
Neoplasm Metastasis
Small Interfering RNA
Cell Line
Animal Models
Lung
Therapeutics
Research

Keywords

  • Adenoid cystic carcinoma
  • Animal model
  • HIF1α
  • Invasion
  • Metastasis

Cite this

Lim, Yun Sung ; Cha, Wonjae ; Park, Min Woo ; Jeong, Woo Jin ; Ahn, Soon-Hyun. / HIF1α in tumorigenesis of adenoid cystic carcinoma. In: Anticancer Research. 2017 ; Vol. 37, No. 2. pp. 599-606.
@article{d240cef8f861430b9f51a0abfbdd0c56,
title = "HIF1α in tumorigenesis of adenoid cystic carcinoma",
abstract = "Background: Tumor hypoxia induces hypoxiainducible factor-1α (HIF1α), which can influence tumorigenesis and metastasis. We evaluated the expression of HIF1α and the effect of HIF1α inhibitors in adenoid cystic carcinoma (ACC). Materials and Methods: HIF1α expression was demonstrated in ACC cell lines (ACC2 and ACCM). The effect of HIF1α inhibitors was evaluated. A systemic metastasis model was developed. The number of metastatic pulmonary nodules were analyzed. Results: The ACCM cell line demonstrated greater HIF1α expression and invasion than ACC2. The expression of HIF1α and invasion of ACC cells were blocked by HIF1α siRNA. HIF1α inhibitors 17-Nallylamino-17-demethoxygeldanamycin (17AAG) and echinomycin inhibited cell invasion. 17AAG inhibited metastasis in the animal model, although not statistically significantly. Conclusion: HIF1α siRNA and 17AAG and echinomycin blocked invasion by ACC2 and ACCM cells. 17AAG exhibited therapeutic potential for inhibition of metastasis. Our results provide positive evidence that HIF1α is a promising research pathway for therapy of ACC.",
keywords = "Adenoid cystic carcinoma, Animal model, HIF1α, Invasion, Metastasis",
author = "Lim, {Yun Sung} and Wonjae Cha and Park, {Min Woo} and Jeong, {Woo Jin} and Soon-Hyun Ahn",
year = "2017",
month = "1",
day = "1",
doi = "10.21873/anticanres.11353",
language = "English",
volume = "37",
pages = "599--606",
journal = "Anticancer research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "2",

}

HIF1α in tumorigenesis of adenoid cystic carcinoma. / Lim, Yun Sung; Cha, Wonjae; Park, Min Woo; Jeong, Woo Jin; Ahn, Soon-Hyun.

In: Anticancer Research, Vol. 37, No. 2, 01.01.2017, p. 599-606.

Research output: Contribution to journalArticle

TY - JOUR

T1 - HIF1α in tumorigenesis of adenoid cystic carcinoma

AU - Lim, Yun Sung

AU - Cha, Wonjae

AU - Park, Min Woo

AU - Jeong, Woo Jin

AU - Ahn, Soon-Hyun

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Background: Tumor hypoxia induces hypoxiainducible factor-1α (HIF1α), which can influence tumorigenesis and metastasis. We evaluated the expression of HIF1α and the effect of HIF1α inhibitors in adenoid cystic carcinoma (ACC). Materials and Methods: HIF1α expression was demonstrated in ACC cell lines (ACC2 and ACCM). The effect of HIF1α inhibitors was evaluated. A systemic metastasis model was developed. The number of metastatic pulmonary nodules were analyzed. Results: The ACCM cell line demonstrated greater HIF1α expression and invasion than ACC2. The expression of HIF1α and invasion of ACC cells were blocked by HIF1α siRNA. HIF1α inhibitors 17-Nallylamino-17-demethoxygeldanamycin (17AAG) and echinomycin inhibited cell invasion. 17AAG inhibited metastasis in the animal model, although not statistically significantly. Conclusion: HIF1α siRNA and 17AAG and echinomycin blocked invasion by ACC2 and ACCM cells. 17AAG exhibited therapeutic potential for inhibition of metastasis. Our results provide positive evidence that HIF1α is a promising research pathway for therapy of ACC.

AB - Background: Tumor hypoxia induces hypoxiainducible factor-1α (HIF1α), which can influence tumorigenesis and metastasis. We evaluated the expression of HIF1α and the effect of HIF1α inhibitors in adenoid cystic carcinoma (ACC). Materials and Methods: HIF1α expression was demonstrated in ACC cell lines (ACC2 and ACCM). The effect of HIF1α inhibitors was evaluated. A systemic metastasis model was developed. The number of metastatic pulmonary nodules were analyzed. Results: The ACCM cell line demonstrated greater HIF1α expression and invasion than ACC2. The expression of HIF1α and invasion of ACC cells were blocked by HIF1α siRNA. HIF1α inhibitors 17-Nallylamino-17-demethoxygeldanamycin (17AAG) and echinomycin inhibited cell invasion. 17AAG inhibited metastasis in the animal model, although not statistically significantly. Conclusion: HIF1α siRNA and 17AAG and echinomycin blocked invasion by ACC2 and ACCM cells. 17AAG exhibited therapeutic potential for inhibition of metastasis. Our results provide positive evidence that HIF1α is a promising research pathway for therapy of ACC.

KW - Adenoid cystic carcinoma

KW - Animal model

KW - HIF1α

KW - Invasion

KW - Metastasis

UR - http://www.scopus.com/inward/record.url?scp=85013666997&partnerID=8YFLogxK

U2 - 10.21873/anticanres.11353

DO - 10.21873/anticanres.11353

M3 - Article

C2 - 28179306

AN - SCOPUS:85013666997

VL - 37

SP - 599

EP - 606

JO - Anticancer research

JF - Anticancer research

SN - 0250-7005

IS - 2

ER -