Heme oxygenase-1 determines the differential response of breast cancer and normal cells to piperlongumine

Ha Na Lee, Hyeon Ok Jin, Jin Ah Park, Jin Hee Kim, Ji Young Kim, Bora Kim, Wonki Kim, Sung Eun Hong, Yun Han Lee, Yoon Hwan Chang, Seok Il Hong, Young Jun Hong, In Chul Park, Young Joon Surh, Jin Kyung Lee

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Piperlongumine, a natural alkaloid isolated from the long pepper, selectively increases reactive oxygen species production and apoptotic cell death in cancer cells but not in normal cells. However, the molecular mechanism underlying piperlongumine-induced selective killing of cancer cells remains unclear. In the present study, we observed that human breast cancer MCF-7 cells are sensitive to piperlongumine-induced apoptosis relative to human MCF-10A breast epithelial cells. Interestingly, this opposing effect of piperlongumine appears to be mediated by heme oxygenase-1 (HO-1). Piperlongumine upregulated HO-1 expression through the activation of nuclear factorerythroid- 2-related factor-2 (Nrf2) signaling in both MCF-7 and MCF-10A cells. However, knockdown of HO-1 expression and pharmacological inhibition of its activity abolished the ability of piperlongumine to induce apoptosis in MCF-7 cells, whereas those promoted apoptosis in MCF-10A cells, indicating that HO-1 has anti-tumor functions in cancer cells but cytoprotective functions in normal cells. Moreover, it was found that piperlongumine-induced Nrf2 activation, HO-1 expression and cancer cell apoptosis are not dependent on the generation of reactive oxygen species. Instead, piperlongumine, which bears electrophilic α,β-unsaturated carbonyl groups, appears to inactivate Kelch-like ECH-associated protein-1 (Keap1) through thiol modification, thereby activating the Nrf2/HO-1 pathway and subsequently upregulating HO-1 expression, which accounts for piperlongumine-induced apoptosis in cancer cells. Taken together, these findings suggest that direct interaction of piperlongumine with Keap1 leads to the upregulation of Nrf2-mediated HO-1 expression, and HO-1 determines the differential response of breast normal cells and cancer cells to piperlongumine.

Original languageEnglish
Pages (from-to)327-335
Number of pages9
JournalMolecules and Cells
Volume38
Issue number4
DOIs
StatePublished - 20 Mar 2015

Keywords

  • Apoptosis
  • Breast cancer
  • HO-1
  • Nrf2
  • Piperlongumine

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    Lee, H. N., Jin, H. O., Park, J. A., Kim, J. H., Kim, J. Y., Kim, B., Kim, W., Hong, S. E., Lee, Y. H., Chang, Y. H., Hong, S. I., Hong, Y. J., Park, I. C., Surh, Y. J., & Lee, J. K. (2015). Heme oxygenase-1 determines the differential response of breast cancer and normal cells to piperlongumine. Molecules and Cells, 38(4), 327-335. https://doi.org/10.14348/molcells.2015.2235