Helicobacter pylori vacuolating toxin exhibits polar activity of Cl- secretion and secretory response to carbachol in T84 cells

Nan Ge Jin, Yong Ri Jin, Insuk So, Ki Whan Kim

Research output: Contribution to journalArticle

Abstract

To investigate whether VacA (vacuolating toxin) produced by Helicobacter pylori Korean stain 99 induces intestinal secretion, purified VacA was added to T84 cell monolayers mounted in Ussing chambers, and electrical parameters were monitored. Mucosal addition of low pH-pretreated VacA increased short circuit current (Isc). The effect was time- and dose-dependent and saturable. The time-to-peak Isc was concentration-dependent. Chloride channel inhibitors, niflumic acid or 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), inhibited VacA-stimulated Isc. Carbachol (CCh)-induced increase of Isc was prolonged by the addition of VacA to the mucosal side only. The effect was unaltered by the addition of niflumic acid. VacA did not show cytopathic effects. These studies indicate that VacA is a nonlethal toxin that acts in a polar manner on T84 monolayers to potentiate Cl- secretion and the response to CCh secretion without decrease in monolayer resistance. VacA may contribute to diarrhea diseases in human intestinal epithelial cells.

Original languageEnglish
Pages (from-to)289-293
Number of pages5
JournalKorean Journal of Physiology and Pharmacology
Volume8
Issue number5
StatePublished - 1 Oct 2004

Keywords

  • Helicobacter pylori
  • Secretion
  • Short circuit current
  • T84 cells
  • VacA

Cite this