Abstract
Objective: To understand the domestication and spread of horses in history, genetic information is essential. However, mitogenetic traits of ancient or medieval horses have yet to be comprehensively revealed, especially for East Asia. This study thus set out to reveal the maternal lineage of skeletal horse remains retrieved from a 15th century archaeological site (Gongpyeongdong) at Old Seoul City in South Korea. Methods: We extracted DNA from the femur of Equus caballus (SNUA001) from Joseon period Gongpyeongdong site. Mitochondrial (mt) DNA (HRS 1512816116) of E. caballus was amplified by polymerase chain reaction. Cloning and sequencing were conducted for the mtDNA amplicons. The sequencing results were analyzed by NCBI/BLAST and phylogenetic tool of MEGA7 software. Results: By means of mtDNA cytochrome b and Dloop analysis, we found that the 15th century Korean horse belonged to haplogroup Q representing those horses that have historically been raised widely in East Asia. Conclusion: The horse is unique among domesticated animals for the remarkable impact it has on human civilization in terms of transportation and trade. Utilizing the Joseonperiod horse remains, we can obtain clues to reveal the genetic traits of Korean horse that existed before the introduction of Western horses.
Original language | English |
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Pages (from-to) | 1141-1150 |
Number of pages | 10 |
Journal | Animal Bioscience |
Volume | 35 |
Issue number | 8 |
DOIs | |
State | Published - 1 Aug 2022 |
Bibliographical note
Publisher Copyright:Copyright © 2022 by Animal Bioscience
Keywords
- Ancient DNA
- Equus caballus
- Horse
- Joseon Period
- Mitochondrial DNA
- South Korea