Forensic SNP Genotyping with SNaPshot: Development of a Novel In-house SBE Multiplex SNP Assay

Mian Sahib Zar, Ahmad Ali Shahid, Muhammad Saqib Shahzad, Kyoung Jin Shin, Hwan Young Lee, Sang Seob Lee, Muhammad Israr, Peter Wiegand, Galina Kulstein

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4 Scopus citations


This study introduces a newly developed in-house SNaPshot single-base extension (SBE) multiplex assay for forensic single nucleotide polymorphism (SNP) genotyping of fresh and degraded samples. The assay was validated with fresh blood samples from four different populations. In addition, altogether 24 samples from skeletal remains were analyzed with the multiplex. Full SNP profiles could be obtained from 14 specimens, while ten remains showed partial SNP profiles. Minor allele frequencies (MAF) of bone samples and different populations were compared and used for association of skeletal remains with a certain population. The results reveal that the SNPs of the bone samples are genetically close to the Pathan population. The findings show that the new multiplex system can be utilized for SNP genotyping of degraded and forensic relevant skeletal material, enabling to provide additional investigative leads in criminal cases.

Original languageEnglish
Pages (from-to)1824-1829
Number of pages6
JournalJournal of Forensic Sciences
Issue number6
StatePublished - Nov 2018


  • forensic genetics
  • forensic genotyping
  • forensic science
  • multiplex development
  • population genetics
  • single nucleotide polymorphism
  • single-base extension
  • skeletal remains

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