Expression of β1 integrin in laminin-adhesion-selected human colon cancer cell lines of varying tumorigenicity

Woo Ho Kim, S. H. Jun, M. C. Kibbey, E. W. Thompson, H. K. Kleinman

Research output: Contribution to journalReview article

24 Citations (Scopus)

Abstract

Laminin has been shown to promote the malignant phenotype and the expression of certain laminin receptors has been correlated with the malignant character of the tumors. Here new cell lines were isolated from a human colon cancer cell line (LCC-C1) based on their adhesiveness to laminin. The laminin-adherent subclone formed large tumors in nude mice, whereas the laminin-nonadherent subclone failed to form sizable tumors. Only the laminin-adherent subclone adhered to laminin and invaded through Matrigel-coated filters. The adhesive and invasive ability of the cells was almost completely blocked by low concentrations (1.0 μg/ml) of anti-β1 integrin antibody. The amounts of total cellular β1 integrin protein were similar in the two subclones when compared by Western blot, and the mRNA levels also did not differ. The localization of β1 integrin laminin receptor varied in the two subclones; the laminin-adherent subclone showed a linear distribution along the cell-cell junctions, while the laminin-nonadherent subclone did not stain between the cells. Using laminin-Sepharose affinity chromatography, more β1 integrin was obtained from the laminin-adherent subclone. These findings suggest that alterations in the affinity of β1 integrin for laminin and in its membrane distribution might be involved in the increased tumorigenicity observed in colon cancer cells.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalInvasion and Metastasis
Volume14
Issue number1-6
StatePublished - 1 Dec 1994

Fingerprint

Laminin
Integrins
Colonic Neoplasms
Cell Line
Laminin Receptors
Adhesiveness
Neoplasms
Agarose Chromatography
Intercellular Junctions
Affinity Chromatography
Nude Mice
Adhesives
Coloring Agents
Western Blotting
Phenotype
Messenger RNA
Membranes
Antibodies

Keywords

  • Colon cancer
  • Invasion
  • Laminin
  • Matrigel
  • β1 Integrin

Cite this

Kim, Woo Ho ; Jun, S. H. ; Kibbey, M. C. ; Thompson, E. W. ; Kleinman, H. K. / Expression of β1 integrin in laminin-adhesion-selected human colon cancer cell lines of varying tumorigenicity. In: Invasion and Metastasis. 1994 ; Vol. 14, No. 1-6. pp. 147-155.
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Expression of β1 integrin in laminin-adhesion-selected human colon cancer cell lines of varying tumorigenicity. / Kim, Woo Ho; Jun, S. H.; Kibbey, M. C.; Thompson, E. W.; Kleinman, H. K.

In: Invasion and Metastasis, Vol. 14, No. 1-6, 01.12.1994, p. 147-155.

Research output: Contribution to journalReview article

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T1 - Expression of β1 integrin in laminin-adhesion-selected human colon cancer cell lines of varying tumorigenicity

AU - Kim, Woo Ho

AU - Jun, S. H.

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AU - Kleinman, H. K.

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AB - Laminin has been shown to promote the malignant phenotype and the expression of certain laminin receptors has been correlated with the malignant character of the tumors. Here new cell lines were isolated from a human colon cancer cell line (LCC-C1) based on their adhesiveness to laminin. The laminin-adherent subclone formed large tumors in nude mice, whereas the laminin-nonadherent subclone failed to form sizable tumors. Only the laminin-adherent subclone adhered to laminin and invaded through Matrigel-coated filters. The adhesive and invasive ability of the cells was almost completely blocked by low concentrations (1.0 μg/ml) of anti-β1 integrin antibody. The amounts of total cellular β1 integrin protein were similar in the two subclones when compared by Western blot, and the mRNA levels also did not differ. The localization of β1 integrin laminin receptor varied in the two subclones; the laminin-adherent subclone showed a linear distribution along the cell-cell junctions, while the laminin-nonadherent subclone did not stain between the cells. Using laminin-Sepharose affinity chromatography, more β1 integrin was obtained from the laminin-adherent subclone. These findings suggest that alterations in the affinity of β1 integrin for laminin and in its membrane distribution might be involved in the increased tumorigenicity observed in colon cancer cells.

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