Abstract
Background and aims: The pathogenesis of coronary artery disease (CAD) is closely associated with chronic inflammatory processes. CD8+ T cells are a key participant in the pathogenesis of atherosclerosis, the major cause of CAD; however, it remains unclear which CD8+ T-cell subset is responsible. We investigated the immunological features of CD8+ T cells expressing low and high levels of the IL-6 receptor α chain (IL-6Rα), a cytokine known to play a key role in cardiovascular diseases. Methods: The expression of IL-6Rα on CD8+ T cells and its association with plasma levels of soluble components of the IL-6/IL-6Rs as well as with clinical parameters were analyzed using FACS analysis and ELISA of CAD patients and age-matched healthy controls (HCs). Immunological characteristics of CD8+ T cells expressing low and high levels of IL-6Rα (CD8+IL-6Rαlow or high) were examined by in vitro culture and intracellular FACS analysis. Results: CAD patients had higher frequencies of circulating CD8+IL-6Rαlow effector memory (EM) T cells compared with HCs (median frequency; 74.59% vs. 60.09%, p = 0.0158). Expanded CD8+IL-6Rαlow T cells positively correlated with the frequency of senescent, cytotoxic CD8+CD57+ T cells (r = 0.6655, p < 0.0001) and plasma IL-6 level (r = 0.3995, p = 0.0432) in CAD patients. Loss of IL-6Rα expression on CD8+ T cells was induced by the combination of IL-6 and IL-15 with accompanying TCR-independent proliferation (p = 0.0101). Moreover, these CD8+IL-6Rαlow T cells had features of type 1 cytotoxic CD8+ T cells. Conclusions: Our findings suggest the possible involvement of expanded CD8+IL-6Rαlow EM T cells in CAD through their pro-inflammatory and highly cytotoxic capacities.
Original language | English |
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Pages (from-to) | 44-51 |
Number of pages | 8 |
Journal | Atherosclerosis |
Volume | 249 |
DOIs | |
State | Published - 1 Jun 2016 |
Bibliographical note
Publisher Copyright:© 2016 Elsevier Ireland Ltd.
Keywords
- CD8 T cells
- Coronary artery disease
- IL-6 receptor α chain
- Interleukin-6
- Type 1 cytotoxic T cells