Enhancement of DNA damage and involvement of reactive oxygen species after exposure to bitumen with UVA irradiation

Yun-Chul Hong, Kwan Hee Lee

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

This study was carried out to evaluate whether bitumen cytotoxicity is enhanced when bitumen treatment is combined with UVA exposure. We also evaluated the oxidative processes in bitumen-induced DNA damage, and attempted to identify the DNA damage caused by bitumen and UVA exposures, either alone or in combination. The effects of bitumen and UVA on cell proliferation were examined using HL 60 cells. DNA-protein crosslinks (DPCs) were assessed using a K-SDS assay, and reactive oxygen species formation was detected by 8-OH-dG formation. We evaluated the formations of double-strand breaks (DSB) using λDNA/HindIII and single-strand breaks (SSB) using PM2 DNA. The cytotoxicity assay showed enhanced suppression of cell proliferation when bitumen exposure and UVA exposure were combined. Combined exposure caused significant increases in DPCs over either exposure alone. Incubation of deoxyguanosine (dG) with bitumen or UVA showed an increase in 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels when compared with controls, and combined exposure enhanced this effect. An evaluation of agarose gel bands showed that DSB and SSB were not formed following exposure to bitumen and UVA. This fact indicates that bitumen and UVA may be involved in genotoxic processes by producing oxygen free radicals and that combined exposure enhances these effects. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume426
Issue number1
DOIs
StatePublished - 3 May 1999

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asphalt
DNA Damage
Reactive Oxygen Species
Deoxyguanosine
DNA
Cell Proliferation
Double-Stranded DNA Breaks
HL-60 Cells

Keywords

  • 8-Hydroxy-2'-deoxyguanosine
  • DNA damage
  • DNA-protein crosslink
  • Double-strand break
  • Reactive oxygen species
  • Single-strand break

Cite this

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title = "Enhancement of DNA damage and involvement of reactive oxygen species after exposure to bitumen with UVA irradiation",
abstract = "This study was carried out to evaluate whether bitumen cytotoxicity is enhanced when bitumen treatment is combined with UVA exposure. We also evaluated the oxidative processes in bitumen-induced DNA damage, and attempted to identify the DNA damage caused by bitumen and UVA exposures, either alone or in combination. The effects of bitumen and UVA on cell proliferation were examined using HL 60 cells. DNA-protein crosslinks (DPCs) were assessed using a K-SDS assay, and reactive oxygen species formation was detected by 8-OH-dG formation. We evaluated the formations of double-strand breaks (DSB) using λDNA/HindIII and single-strand breaks (SSB) using PM2 DNA. The cytotoxicity assay showed enhanced suppression of cell proliferation when bitumen exposure and UVA exposure were combined. Combined exposure caused significant increases in DPCs over either exposure alone. Incubation of deoxyguanosine (dG) with bitumen or UVA showed an increase in 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels when compared with controls, and combined exposure enhanced this effect. An evaluation of agarose gel bands showed that DSB and SSB were not formed following exposure to bitumen and UVA. This fact indicates that bitumen and UVA may be involved in genotoxic processes by producing oxygen free radicals and that combined exposure enhances these effects. Copyright (C) 1999 Elsevier Science B.V.",
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Enhancement of DNA damage and involvement of reactive oxygen species after exposure to bitumen with UVA irradiation. / Hong, Yun-Chul; Lee, Kwan Hee.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 426, No. 1, 03.05.1999, p. 63-69.

Research output: Contribution to journalArticleResearchpeer-review

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