Engineered cartilage utilizing fetal cartilage-derived progenitor cells for cartilage repair

Do Young Park, Byoung Hyun Min, So Ra Park, Hyun Ju Oh, Minh Dung Truong, Mijin Kim, Ja Young Choi, In Su Park, Byung Hyune Choi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The aim of this study was to develop a fetal cartilage-derived progenitor cell (FCPC) based cartilage gel through self-assembly for cartilage repair surgery, with clinically useful properties including adhesiveness, plasticity, and continued chondrogenic remodeling after transplantation. Characterization of the gels according to in vitro self-assembly period resulted in increased chondrogenic features over time. Adhesion strength of the cartilage gels were significantly higher compared to alginate gel, with the 2-wk group showing a near 20-fold higher strength (1.8 ± 0.15 kPa vs. 0.09 ± 0.01 kPa, p < 0.001). The in vivo remodeling process analysis of the 2 wk cultured gels showed increased cartilage repair characteristics and stiffness over time, with higher integration-failure stress compared to osteochondral autograft controls at 4 weeks (p < 0.01). In the nonhuman primate investigation, cartilage repair scores were significantly better in the gel group compared to defects alone after 24 weeks (p < 0.001). Cell distribution analysis at 24 weeks showed that human cells remained within the transplanted defects only. A self-assembled, FCPC-based cartilage gel showed chondrogenic repair potential as well as adhesive properties, beneficial for cartilage repair.

Original languageEnglish
Article number5722
JournalScientific Reports
Volume10
Issue number1
DOIs
StatePublished - 1 Dec 2020

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