Elevation of CRABP-I in the Cerebrospinal Fluid of Patients with Moyamoya Disease

Seung Ki Kim, Jong Il Yoo, Byung Kyu Cho, Soo Jin Hong, Yong Kook Kim, Jung Ae Moon, Ji Ha Kim, You Nam Chung, Kyu Chang Wang

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Abstract

Background and Purpose-The etiology of moyamoya disease (MMD) remains obscure. This study was undertaken to identify specific proteins associated with the pathogenesis of MMD. Methods-We studied cerebrospinal fluid (CSF) from 20 patients with angiographically confirmed MMD (4 boys and 16 girls; age range, 3 to 13 years; mean, 7.5 years) and 4 control patients with cerebral palsy who underwent selective dorsal rhizotomy (2 boys and 2 girls; age range, 5 to 10 years; mean, 7.3 years). CSF proteins were analyzed by 2-dimensional polyacrylamide gel electrophoresis, and protein identification was performed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presence of specific CSF protein in patients with MMD was confirmed by Western blotting. In addition, cerebral CSF was also tested in 7 patients who had other brain diseases but no MMD (2 boys and 5 girls; age range, 1 to 12 years; mean, 6.9 years). Results-We identified 1 polypeptide spot (Mr of 13 to 15 kDa and isoelectric point of 5 to 5.5) that was differentially expressed in the CSF samples of MMD patients (mean optical density intensity, 0.36±0.24; range, 0.05 to 0.92) and control spinal CSF samples (mean, 0.03±0.04; range, 0 to 0.08; P=0.002). This polypeptide was identified as cellular retinoic acid-binding protein (CRABP)-I. High levels of expression of CRABP-I in the CSF from 17 MMD children were confirmed by Western blotting. Conclusions-The analysis of the CSF of MMD patients reveals high CRABP-I expression. The present study suggests that the elevation of CRABP-I in CSF may be a candidate for pathogenesis of MMD.

Original languageEnglish
Pages (from-to)2835-2841
Number of pages7
JournalStroke
Volume34
Issue number12
DOIs
StatePublished - 1 Dec 2003

Keywords

  • Cerebrospinal fluid
  • Electrophoresis, gel, two-dimensional
  • Moyamoya disease
  • Protein binding
  • Receptors, retinoic acid
  • Spectrometry, mass, matrix-assisted laser desorption-ionization

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    Kim, S. K., Yoo, J. I., Cho, B. K., Hong, S. J., Kim, Y. K., Moon, J. A., Kim, J. H., Chung, Y. N., & Wang, K. C. (2003). Elevation of CRABP-I in the Cerebrospinal Fluid of Patients with Moyamoya Disease. Stroke, 34(12), 2835-2841. https://doi.org/10.1161/01.STR.0000100159.43123.D7