Effects of prostaglandin e2 on the spontaneous contractions and electrical activities of the antral circular muscle in guinea-pig stomach

Yoon Kim Jong Yoon Kim, Sungjoon Kim, Mook Kang Tong Mook Kang, Jin Lee Sang Jin Lee, Yeoul Jun Jae Yeoul Jun, Insuk So, Whan Kim Ki Whan Kim

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The spontaneous contractions of gastric smooth muscles are regulated by slow waves, which are modulated by both nervous system and humoral agents. This study was designed to examine the effects of prostaglandin E2 (PGF2) on the contractile and electrical activities of antral smooth muscles in guinea-pig stomach, using an intracellular recording technique. To elucidate the underlying mechanism for its effect on contractility, ionic currents were also measured using a whole-cell patch clamp method. The basal tone by PGE2 was variable, whereas the magnitude of phasic contractions was reduced (19.0±2.1%, n=19). The resting membrane potentials were hyperpolarized (- 4.4 ± 0.5 mV, n=10), and plateau potentials were lowered (- 2.9 ± 0.5 mV, n=10). In most cases, however, the initial peak potentials of slow waves were depolarized more by PGE2 than those of control. The frequency of the slows wave was increased from 5.7±0.2 cycles/min to 6.5±0.2 (n=22). Voltage- operated Ca2+ currents were decreased by PGF2 (n=5). Voltage-operated K+ currents, both Ca-dependent and Ca-independent, were increased (n=5). These results suggest that PGE2 plays an important role in the modulation of gastric smooth muscle activities, and its inhibitory effects on the contractility and activities of slow waves are resulted from both decrease of Ca2+ currents and increase of K+ currents.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalKorean Journal of Physiology and Pharmacology
Volume2
Issue number3
StatePublished - 1 Jan 1998

Fingerprint

Dinoprostone
Stomach
Guinea Pigs
Smooth Muscle
Muscles
Dinoprost
Membrane Potentials
Nervous System
Antral

Keywords

  • Ca current
  • Gastric smooth muscle
  • K current
  • PGE
  • Slow waves

Cite this

Jong Yoon Kim, Y. K., Kim, S., Tong Mook Kang, M. K., Sang Jin Lee, J. L., Jae Yeoul Jun, Y. J., So, I., & Ki Whan Kim, W. K. (1998). Effects of prostaglandin e2 on the spontaneous contractions and electrical activities of the antral circular muscle in guinea-pig stomach. Korean Journal of Physiology and Pharmacology, 2(3), 361-368.
Jong Yoon Kim, Yoon Kim ; Kim, Sungjoon ; Tong Mook Kang, Mook Kang ; Sang Jin Lee, Jin Lee ; Jae Yeoul Jun, Yeoul Jun ; So, Insuk ; Ki Whan Kim, Whan Kim. / Effects of prostaglandin e2 on the spontaneous contractions and electrical activities of the antral circular muscle in guinea-pig stomach. In: Korean Journal of Physiology and Pharmacology. 1998 ; Vol. 2, No. 3. pp. 361-368.
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Jong Yoon Kim, YK, Kim, S, Tong Mook Kang, MK, Sang Jin Lee, JL, Jae Yeoul Jun, YJ, So, I & Ki Whan Kim, WK 1998, 'Effects of prostaglandin e2 on the spontaneous contractions and electrical activities of the antral circular muscle in guinea-pig stomach', Korean Journal of Physiology and Pharmacology, vol. 2, no. 3, pp. 361-368.

Effects of prostaglandin e2 on the spontaneous contractions and electrical activities of the antral circular muscle in guinea-pig stomach. / Jong Yoon Kim, Yoon Kim; Kim, Sungjoon; Tong Mook Kang, Mook Kang; Sang Jin Lee, Jin Lee; Jae Yeoul Jun, Yeoul Jun; So, Insuk; Ki Whan Kim, Whan Kim.

In: Korean Journal of Physiology and Pharmacology, Vol. 2, No. 3, 01.01.1998, p. 361-368.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Effects of prostaglandin e2 on the spontaneous contractions and electrical activities of the antral circular muscle in guinea-pig stomach

AU - Jong Yoon Kim, Yoon Kim

AU - Kim, Sungjoon

AU - Tong Mook Kang, Mook Kang

AU - Sang Jin Lee, Jin Lee

AU - Jae Yeoul Jun, Yeoul Jun

AU - So, Insuk

AU - Ki Whan Kim, Whan Kim

PY - 1998/1/1

Y1 - 1998/1/1

N2 - The spontaneous contractions of gastric smooth muscles are regulated by slow waves, which are modulated by both nervous system and humoral agents. This study was designed to examine the effects of prostaglandin E2 (PGF2) on the contractile and electrical activities of antral smooth muscles in guinea-pig stomach, using an intracellular recording technique. To elucidate the underlying mechanism for its effect on contractility, ionic currents were also measured using a whole-cell patch clamp method. The basal tone by PGE2 was variable, whereas the magnitude of phasic contractions was reduced (19.0±2.1%, n=19). The resting membrane potentials were hyperpolarized (- 4.4 ± 0.5 mV, n=10), and plateau potentials were lowered (- 2.9 ± 0.5 mV, n=10). In most cases, however, the initial peak potentials of slow waves were depolarized more by PGE2 than those of control. The frequency of the slows wave was increased from 5.7±0.2 cycles/min to 6.5±0.2 (n=22). Voltage- operated Ca2+ currents were decreased by PGF2 (n=5). Voltage-operated K+ currents, both Ca-dependent and Ca-independent, were increased (n=5). These results suggest that PGE2 plays an important role in the modulation of gastric smooth muscle activities, and its inhibitory effects on the contractility and activities of slow waves are resulted from both decrease of Ca2+ currents and increase of K+ currents.

AB - The spontaneous contractions of gastric smooth muscles are regulated by slow waves, which are modulated by both nervous system and humoral agents. This study was designed to examine the effects of prostaglandin E2 (PGF2) on the contractile and electrical activities of antral smooth muscles in guinea-pig stomach, using an intracellular recording technique. To elucidate the underlying mechanism for its effect on contractility, ionic currents were also measured using a whole-cell patch clamp method. The basal tone by PGE2 was variable, whereas the magnitude of phasic contractions was reduced (19.0±2.1%, n=19). The resting membrane potentials were hyperpolarized (- 4.4 ± 0.5 mV, n=10), and plateau potentials were lowered (- 2.9 ± 0.5 mV, n=10). In most cases, however, the initial peak potentials of slow waves were depolarized more by PGE2 than those of control. The frequency of the slows wave was increased from 5.7±0.2 cycles/min to 6.5±0.2 (n=22). Voltage- operated Ca2+ currents were decreased by PGF2 (n=5). Voltage-operated K+ currents, both Ca-dependent and Ca-independent, were increased (n=5). These results suggest that PGE2 plays an important role in the modulation of gastric smooth muscle activities, and its inhibitory effects on the contractility and activities of slow waves are resulted from both decrease of Ca2+ currents and increase of K+ currents.

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KW - Gastric smooth muscle

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