Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium

Hainan Piao, Taejin Youn, Jin Sook Kwon, Young Hwa Kim, Jang Whan Bae, Bora-Sohn, Dong Woon Kim, Myeong Chan Cho, Myoung Mook Lee, Young Bae Park

Research output: Contribution to journalArticle

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Abstract

Background: Cellular cardiomyoplasty (CCM) is considered to be a novel therapeutic approach for post-myocardial infarction (MI) heart failure. In this study, the functional effects of cultured mesenchymal stem cells (MSCs) transplantation and the associated histopathologic changes were evaluated in a rat model of MI. Methods: Rats were subjected to 5 h of coronary ligation followed by reperfusion and, 10 days after MI, animals were randomized into either the MSCs transplantation (MI-MSC, n=8) group or the control (n=8) group. Allogeneic MSCs (3×106 cells) or media were epicardially injected into the center and the border area of the infarct scar. Results: Four weeks after the MSCs transplantation, the echocardiogram showed preserved anterior regional wall motion and increases in fractional shortening in the MI-MSC heart relative to the control heart. Left ventricular (LV) end-diastolic pressure was smaller in the MI-MSC than in the control group. Implanted MSCs formed islands of cell clusters on the border of the infarct scar, and the cells were positively immunostained by sarcomeric α-actinin and cardiac troponin T. In addition, the number of microvessels on the border area of the infarct scar was greater in the MI-MSC than in the control group. Conclusion: Allogeneic MSCs transplanted into the MI scar formed clusters of cell grafts on the border of the infarct, expressed cardiac muscle proteins, increased microvessel formation, and improved regional and global LV function. Our data indicate that CCM using MSCs may have a significant role in the treatment of post-MI heart failure.

Original languageEnglish
Pages (from-to)730-738
Number of pages9
JournalEuropean Journal of Heart Failure
Volume7
Issue number5
DOIs
StatePublished - 1 Aug 2005

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Mesenchymal Stem Cell Transplantation
Mesenchymal Stromal Cells
Myocardium
Bone Marrow
Myocardial Infarction
Cicatrix
Cardiomyoplasty
Microvessels
Control Groups
Heart Failure
Actinin
Troponin T
Muscle Proteins
Left Ventricular Function
Reperfusion
Ligation
Blood Pressure
Transplants

Keywords

  • Cells
  • Heart failure
  • Myocardial infarction
  • Transplantation

Cite this

Piao, Hainan ; Youn, Taejin ; Kwon, Jin Sook ; Kim, Young Hwa ; Bae, Jang Whan ; Bora-Sohn ; Kim, Dong Woon ; Cho, Myeong Chan ; Lee, Myoung Mook ; Park, Young Bae. / Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium. In: European Journal of Heart Failure. 2005 ; Vol. 7, No. 5. pp. 730-738.
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abstract = "Background: Cellular cardiomyoplasty (CCM) is considered to be a novel therapeutic approach for post-myocardial infarction (MI) heart failure. In this study, the functional effects of cultured mesenchymal stem cells (MSCs) transplantation and the associated histopathologic changes were evaluated in a rat model of MI. Methods: Rats were subjected to 5 h of coronary ligation followed by reperfusion and, 10 days after MI, animals were randomized into either the MSCs transplantation (MI-MSC, n=8) group or the control (n=8) group. Allogeneic MSCs (3×106 cells) or media were epicardially injected into the center and the border area of the infarct scar. Results: Four weeks after the MSCs transplantation, the echocardiogram showed preserved anterior regional wall motion and increases in fractional shortening in the MI-MSC heart relative to the control heart. Left ventricular (LV) end-diastolic pressure was smaller in the MI-MSC than in the control group. Implanted MSCs formed islands of cell clusters on the border of the infarct scar, and the cells were positively immunostained by sarcomeric α-actinin and cardiac troponin T. In addition, the number of microvessels on the border area of the infarct scar was greater in the MI-MSC than in the control group. Conclusion: Allogeneic MSCs transplanted into the MI scar formed clusters of cell grafts on the border of the infarct, expressed cardiac muscle proteins, increased microvessel formation, and improved regional and global LV function. Our data indicate that CCM using MSCs may have a significant role in the treatment of post-MI heart failure.",
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Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium. / Piao, Hainan; Youn, Taejin; Kwon, Jin Sook; Kim, Young Hwa; Bae, Jang Whan; Bora-Sohn; Kim, Dong Woon; Cho, Myeong Chan; Lee, Myoung Mook; Park, Young Bae.

In: European Journal of Heart Failure, Vol. 7, No. 5, 01.08.2005, p. 730-738.

Research output: Contribution to journalArticle

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T1 - Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium

AU - Piao, Hainan

AU - Youn, Taejin

AU - Kwon, Jin Sook

AU - Kim, Young Hwa

AU - Bae, Jang Whan

AU - Bora-Sohn,

AU - Kim, Dong Woon

AU - Cho, Myeong Chan

AU - Lee, Myoung Mook

AU - Park, Young Bae

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Y1 - 2005/8/1

N2 - Background: Cellular cardiomyoplasty (CCM) is considered to be a novel therapeutic approach for post-myocardial infarction (MI) heart failure. In this study, the functional effects of cultured mesenchymal stem cells (MSCs) transplantation and the associated histopathologic changes were evaluated in a rat model of MI. Methods: Rats were subjected to 5 h of coronary ligation followed by reperfusion and, 10 days after MI, animals were randomized into either the MSCs transplantation (MI-MSC, n=8) group or the control (n=8) group. Allogeneic MSCs (3×106 cells) or media were epicardially injected into the center and the border area of the infarct scar. Results: Four weeks after the MSCs transplantation, the echocardiogram showed preserved anterior regional wall motion and increases in fractional shortening in the MI-MSC heart relative to the control heart. Left ventricular (LV) end-diastolic pressure was smaller in the MI-MSC than in the control group. Implanted MSCs formed islands of cell clusters on the border of the infarct scar, and the cells were positively immunostained by sarcomeric α-actinin and cardiac troponin T. In addition, the number of microvessels on the border area of the infarct scar was greater in the MI-MSC than in the control group. Conclusion: Allogeneic MSCs transplanted into the MI scar formed clusters of cell grafts on the border of the infarct, expressed cardiac muscle proteins, increased microvessel formation, and improved regional and global LV function. Our data indicate that CCM using MSCs may have a significant role in the treatment of post-MI heart failure.

AB - Background: Cellular cardiomyoplasty (CCM) is considered to be a novel therapeutic approach for post-myocardial infarction (MI) heart failure. In this study, the functional effects of cultured mesenchymal stem cells (MSCs) transplantation and the associated histopathologic changes were evaluated in a rat model of MI. Methods: Rats were subjected to 5 h of coronary ligation followed by reperfusion and, 10 days after MI, animals were randomized into either the MSCs transplantation (MI-MSC, n=8) group or the control (n=8) group. Allogeneic MSCs (3×106 cells) or media were epicardially injected into the center and the border area of the infarct scar. Results: Four weeks after the MSCs transplantation, the echocardiogram showed preserved anterior regional wall motion and increases in fractional shortening in the MI-MSC heart relative to the control heart. Left ventricular (LV) end-diastolic pressure was smaller in the MI-MSC than in the control group. Implanted MSCs formed islands of cell clusters on the border of the infarct scar, and the cells were positively immunostained by sarcomeric α-actinin and cardiac troponin T. In addition, the number of microvessels on the border area of the infarct scar was greater in the MI-MSC than in the control group. Conclusion: Allogeneic MSCs transplanted into the MI scar formed clusters of cell grafts on the border of the infarct, expressed cardiac muscle proteins, increased microvessel formation, and improved regional and global LV function. Our data indicate that CCM using MSCs may have a significant role in the treatment of post-MI heart failure.

KW - Cells

KW - Heart failure

KW - Myocardial infarction

KW - Transplantation

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