Effect of stretch on calcium channel currents recorded from the antral circular myocytes of guinea pig stomach

Wen Xie Xu, Sungjoon Kim, Sangjeong Kim, Insuk So, Tong Mook Kang, Jong Chul Rhee, Ki Whan Kim

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Abstract

The effect of membrane stretch on voltage-activated Ba2+ current (I(Ba)) was studied in antral circular myocytes of guinea-pig using the whole-cell patch-clamp technique. The changes in cell volume were elicited by superfusing the myocytes with anisosmotic solutions. Hyposmotic superfusate (202 mosmol/l) induced cell swelling and increased peak values of I(Ba), at 0 mV (from -406.6±45.5 pA to -547.5±65.6 pA, mean±SEM, n=8) and hyperosmotic superfusate (350 mosmol/l) induced cell shrinkage and decreased peak values of I(Ba), at 0 mV (to -269.5±39.1 pA, n=8). Such changes were reversible and the extent of change was dependent on the osmolarity of superfusate. The values of normalized I(Ba) at 0 mV were 1.43±0.04, 1.30±0.06, 1.23±0.04, 1.19±0.04, 1 and 0.68±0.06 at 202, 220, 245, 267, 290 and 350 mosmol/l, respectively (n=8). I(Ba) was almost completely blocked by nicardipine (5 μM) under hyposmotic conditions. The values of steady-state haIf-inactivation voltage (-37.7±3.3 and -36.5±2.6 mV, under control and hyposmotic conditions, respectively) or the half-activation voltage (-13.6±2.3 and -13.9±1.9 mV) of I(Ba) were not significantly changed (P>0.05, n=6). Cell membrane capacitance was slightly increased from 50.00±2.86 pF to 50.22±2.82 pF by a hyposmotic superfusate (P<0.05, n=6). It is suggested that cell swelling increases voltage-operated L-type calcium channel current and that such a property is related to the response of gastric smooth muscle to mechanical stimuli.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalPflugers Archiv European Journal of Physiology
Volume432
Issue number2
DOIs
StatePublished - 19 Jul 1996

Fingerprint

Calcium Channels
Muscle Cells
Stomach
Guinea Pigs
Nicardipine
L-Type Calcium Channels
Patch-Clamp Techniques
Cell Size
Osmolar Concentration
Smooth Muscle
Cell Membrane
Membranes
Antral

Keywords

  • Ca channel current
  • Gastric myocytes
  • Membrane capacitance
  • Stretch

Cite this

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title = "Effect of stretch on calcium channel currents recorded from the antral circular myocytes of guinea pig stomach",
abstract = "The effect of membrane stretch on voltage-activated Ba2+ current (I(Ba)) was studied in antral circular myocytes of guinea-pig using the whole-cell patch-clamp technique. The changes in cell volume were elicited by superfusing the myocytes with anisosmotic solutions. Hyposmotic superfusate (202 mosmol/l) induced cell swelling and increased peak values of I(Ba), at 0 mV (from -406.6±45.5 pA to -547.5±65.6 pA, mean±SEM, n=8) and hyperosmotic superfusate (350 mosmol/l) induced cell shrinkage and decreased peak values of I(Ba), at 0 mV (to -269.5±39.1 pA, n=8). Such changes were reversible and the extent of change was dependent on the osmolarity of superfusate. The values of normalized I(Ba) at 0 mV were 1.43±0.04, 1.30±0.06, 1.23±0.04, 1.19±0.04, 1 and 0.68±0.06 at 202, 220, 245, 267, 290 and 350 mosmol/l, respectively (n=8). I(Ba) was almost completely blocked by nicardipine (5 μM) under hyposmotic conditions. The values of steady-state haIf-inactivation voltage (-37.7±3.3 and -36.5±2.6 mV, under control and hyposmotic conditions, respectively) or the half-activation voltage (-13.6±2.3 and -13.9±1.9 mV) of I(Ba) were not significantly changed (P>0.05, n=6). Cell membrane capacitance was slightly increased from 50.00±2.86 pF to 50.22±2.82 pF by a hyposmotic superfusate (P<0.05, n=6). It is suggested that cell swelling increases voltage-operated L-type calcium channel current and that such a property is related to the response of gastric smooth muscle to mechanical stimuli.",
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Effect of stretch on calcium channel currents recorded from the antral circular myocytes of guinea pig stomach. / Xu, Wen Xie; Kim, Sungjoon; Kim, Sangjeong; So, Insuk; Kang, Tong Mook; Rhee, Jong Chul; Kim, Ki Whan.

In: Pflugers Archiv European Journal of Physiology, Vol. 432, No. 2, 19.07.1996, p. 159-164.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Effect of stretch on calcium channel currents recorded from the antral circular myocytes of guinea pig stomach

AU - Xu, Wen Xie

AU - Kim, Sungjoon

AU - Kim, Sangjeong

AU - So, Insuk

AU - Kang, Tong Mook

AU - Rhee, Jong Chul

AU - Kim, Ki Whan

PY - 1996/7/19

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N2 - The effect of membrane stretch on voltage-activated Ba2+ current (I(Ba)) was studied in antral circular myocytes of guinea-pig using the whole-cell patch-clamp technique. The changes in cell volume were elicited by superfusing the myocytes with anisosmotic solutions. Hyposmotic superfusate (202 mosmol/l) induced cell swelling and increased peak values of I(Ba), at 0 mV (from -406.6±45.5 pA to -547.5±65.6 pA, mean±SEM, n=8) and hyperosmotic superfusate (350 mosmol/l) induced cell shrinkage and decreased peak values of I(Ba), at 0 mV (to -269.5±39.1 pA, n=8). Such changes were reversible and the extent of change was dependent on the osmolarity of superfusate. The values of normalized I(Ba) at 0 mV were 1.43±0.04, 1.30±0.06, 1.23±0.04, 1.19±0.04, 1 and 0.68±0.06 at 202, 220, 245, 267, 290 and 350 mosmol/l, respectively (n=8). I(Ba) was almost completely blocked by nicardipine (5 μM) under hyposmotic conditions. The values of steady-state haIf-inactivation voltage (-37.7±3.3 and -36.5±2.6 mV, under control and hyposmotic conditions, respectively) or the half-activation voltage (-13.6±2.3 and -13.9±1.9 mV) of I(Ba) were not significantly changed (P>0.05, n=6). Cell membrane capacitance was slightly increased from 50.00±2.86 pF to 50.22±2.82 pF by a hyposmotic superfusate (P<0.05, n=6). It is suggested that cell swelling increases voltage-operated L-type calcium channel current and that such a property is related to the response of gastric smooth muscle to mechanical stimuli.

AB - The effect of membrane stretch on voltage-activated Ba2+ current (I(Ba)) was studied in antral circular myocytes of guinea-pig using the whole-cell patch-clamp technique. The changes in cell volume were elicited by superfusing the myocytes with anisosmotic solutions. Hyposmotic superfusate (202 mosmol/l) induced cell swelling and increased peak values of I(Ba), at 0 mV (from -406.6±45.5 pA to -547.5±65.6 pA, mean±SEM, n=8) and hyperosmotic superfusate (350 mosmol/l) induced cell shrinkage and decreased peak values of I(Ba), at 0 mV (to -269.5±39.1 pA, n=8). Such changes were reversible and the extent of change was dependent on the osmolarity of superfusate. The values of normalized I(Ba) at 0 mV were 1.43±0.04, 1.30±0.06, 1.23±0.04, 1.19±0.04, 1 and 0.68±0.06 at 202, 220, 245, 267, 290 and 350 mosmol/l, respectively (n=8). I(Ba) was almost completely blocked by nicardipine (5 μM) under hyposmotic conditions. The values of steady-state haIf-inactivation voltage (-37.7±3.3 and -36.5±2.6 mV, under control and hyposmotic conditions, respectively) or the half-activation voltage (-13.6±2.3 and -13.9±1.9 mV) of I(Ba) were not significantly changed (P>0.05, n=6). Cell membrane capacitance was slightly increased from 50.00±2.86 pF to 50.22±2.82 pF by a hyposmotic superfusate (P<0.05, n=6). It is suggested that cell swelling increases voltage-operated L-type calcium channel current and that such a property is related to the response of gastric smooth muscle to mechanical stimuli.

KW - Ca channel current

KW - Gastric myocytes

KW - Membrane capacitance

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