Effect of porosity and phase composition in 3D printed calcium phosphate scaffolds on bone tissue regeneration in vivo

Naren Raja, Shi Huan Han, Minjoon Cho, Yeong Jin Choi, Yuan Zhe Jin, Honghyun Park, Jae Hyup Lee, Hui suk Yun

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Highly porous calcium phosphate (CaP) scaffolds with multiple phases were fabricated through combination of material extrusion type 3D printing process, salt-leaching, and bone cement chemistry under physiological conditions. Scaffolds with nano-sized calcium deficient hydroxy apatite (CDHA) in the core and large crystals of dicalcium phosphate dihydrate (DCPD or brushite) on the outer surface were fabricated by immersing the 3D ceramic scaffold in aqueous ‘cement solution’. In contrast to conventional CaP cement reactions where a single product is obtained, here we could achieve a composite of apatite-brushite. The composite scaffolds showed improved compressive strength without loss of porosity which was suitable for efficient bone tissue regeneration. With 65 % porosity, these scaffolds showed compressive strength of 15 MPa. The porosity of CaP scaffold was increased by applying salt-leaching technique to 3D printing, which greatly influenced the biodegradation of the scaffold in vivo. Composite scaffolds with fast degrading brushite shell showed better and faster scaffold-to-bone integration compared to apatite based scaffold. In vivo studies verified that multi-phase CaP scaffolds with bimodal pore structure induced good new bone formation behavior (85 % of coverage rate on calvaria defect site after 11 weeks of implantation) with good biodegradability (76.6 % of degradation in rabbit subdermal model after 12 weeks of implantation).

Original languageEnglish
Article number110819
JournalMaterials and Design
StatePublished - Jul 2022


  • 3D printing
  • Biodegradability
  • Bone tissue regeneration
  • Cement reaction
  • Multi-phase calcium phosphates
  • Salt-leaching


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