Effect of 8-methoxypsoralen on PHA-induced DNA synthesis of human lymphocyte in the absence of UVA

J. H. Chung, K. H. Kim, J. I. Youn, J. H. Ree

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We have observed the dark effect of 8-methoxypsoralen (8-MOP) on the viability and DNA synthesis in human lymphocyte cultures after stimulation with phytohemagglutinin (PHA) in the absence of ultraviolet A radiation. The concentrations of 8-MOP was 0.5-32 μg/ml. We have also measured the LDH activity in supernatants of lymphocyte cultures treated with 8-MOP. The results were as follows: 1. Thsere was no 8-MOP dose-dependent decrease in the viability of lymphocytes up to 8-MOP 32 μg/ml. 2. There was a 8-MOP dose-dependent decrease in PHA-induced DNA synthesis of lymphocytes from the concentration of 8-MOP 2 μg/ml. 3. There was a time-dependent decrease in PHA-induced DNA synthesis of lymphocytes at the concentration of 8-MOP 32 μg/ml. 4. There were no LDH release in supernatant of lymphocyte cultures after incubation with 8-MOP up to 8-MOP 32 μg/ml.

Original languageEnglish
Pages (from-to)601-607
Number of pages7
JournalKorean Journal of Dermatology
Issue number5
StatePublished - 1 Dec 1992

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