Ectopic expression of Neuronatin potentiates adipogenesis through enhanced phosphorylation of cAMP-response element-binding protein in 3T3-L1 cells

Young Ho Suh, Ho Kim Won, Changsuk Moon, Hwa Hong Yun, Su Yong Eun, Hyun Lim Joo, Sun Choi Joo, Jihyun Song, Myeong Ho Jung

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Neuronatin (Nnat) is selectively expressed in the neonatal brain and is involved in neuronal differentiation during brain development. However, Nnat also appears to be abundantly expressed in adipose tissue, and is conspicuously elevated in the adipose tissue of obese Zucker diabetic fatty rats compared with control lean Zucker lean control rats shown in our previous report. Here, we examined the expression of Nnat in adipose tissue and demonstrated that the ectopic expression of Nnat mediated by retroviral infection or stable transfection of 3T3-L1 pre-adipocytes stimulated differentiation into mature adipocytes with early induction of adipogenic transcription factors. Moreover, in 3T3-L1 cells overexpressing Nnat, increased intracellular free calcium levels and enhanced phosphorylation of cAMP-response element-binding protein (CREB) were observed, which appears to potentiate CCAAT/enhancer-binding protein (C/EBP)β, C/EBPδ, and C/EBPα transcriptional activities. Collectively, the data indicate that Nnat enhances CREB phosphorylation through increasing intracellular free calcium levels, which potentiates expression of adipogenic transcription factors resulting in heightened adipocyte differentiation. These findings contribute to a greater fundamental understanding of obesity, a clinically important risk factor in numerous diseases.

Original languageEnglish
Pages (from-to)481-489
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume337
Issue number2
DOIs
StatePublished - 18 Nov 2005

Fingerprint

CCAAT-Enhancer-Binding Proteins
3T3-L1 Cells
Cyclic AMP Response Element-Binding Protein
Adipogenesis
Phosphorylation
Adipocytes
Adipose Tissue
Tissue
Brain
Transcription Factors
Rat control
Calcium
Transfection
Rats
Obesity
Infection
Ectopic Gene Expression

Keywords

  • 3T3-L1
  • Adipocyte differentiation
  • CREB
  • Intracellular calcium
  • Neuronatin

Cite this

Suh, Young Ho ; Won, Ho Kim ; Moon, Changsuk ; Yun, Hwa Hong ; Eun, Su Yong ; Joo, Hyun Lim ; Joo, Sun Choi ; Song, Jihyun ; Jung, Myeong Ho. / Ectopic expression of Neuronatin potentiates adipogenesis through enhanced phosphorylation of cAMP-response element-binding protein in 3T3-L1 cells. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 337, No. 2. pp. 481-489.
@article{9a1cdd310b51443a99271686552fba54,
title = "Ectopic expression of Neuronatin potentiates adipogenesis through enhanced phosphorylation of cAMP-response element-binding protein in 3T3-L1 cells",
abstract = "Neuronatin (Nnat) is selectively expressed in the neonatal brain and is involved in neuronal differentiation during brain development. However, Nnat also appears to be abundantly expressed in adipose tissue, and is conspicuously elevated in the adipose tissue of obese Zucker diabetic fatty rats compared with control lean Zucker lean control rats shown in our previous report. Here, we examined the expression of Nnat in adipose tissue and demonstrated that the ectopic expression of Nnat mediated by retroviral infection or stable transfection of 3T3-L1 pre-adipocytes stimulated differentiation into mature adipocytes with early induction of adipogenic transcription factors. Moreover, in 3T3-L1 cells overexpressing Nnat, increased intracellular free calcium levels and enhanced phosphorylation of cAMP-response element-binding protein (CREB) were observed, which appears to potentiate CCAAT/enhancer-binding protein (C/EBP)β, C/EBPδ, and C/EBPα transcriptional activities. Collectively, the data indicate that Nnat enhances CREB phosphorylation through increasing intracellular free calcium levels, which potentiates expression of adipogenic transcription factors resulting in heightened adipocyte differentiation. These findings contribute to a greater fundamental understanding of obesity, a clinically important risk factor in numerous diseases.",
keywords = "3T3-L1, Adipocyte differentiation, CREB, Intracellular calcium, Neuronatin",
author = "Suh, {Young Ho} and Won, {Ho Kim} and Changsuk Moon and Yun, {Hwa Hong} and Eun, {Su Yong} and Joo, {Hyun Lim} and Joo, {Sun Choi} and Jihyun Song and Jung, {Myeong Ho}",
year = "2005",
month = "11",
day = "18",
doi = "10.1016/j.bbrc.2005.09.078",
language = "English",
volume = "337",
pages = "481--489",
journal = "Biochemical and biophysical research communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

Ectopic expression of Neuronatin potentiates adipogenesis through enhanced phosphorylation of cAMP-response element-binding protein in 3T3-L1 cells. / Suh, Young Ho; Won, Ho Kim; Moon, Changsuk; Yun, Hwa Hong; Eun, Su Yong; Joo, Hyun Lim; Joo, Sun Choi; Song, Jihyun; Jung, Myeong Ho.

In: Biochemical and Biophysical Research Communications, Vol. 337, No. 2, 18.11.2005, p. 481-489.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Ectopic expression of Neuronatin potentiates adipogenesis through enhanced phosphorylation of cAMP-response element-binding protein in 3T3-L1 cells

AU - Suh, Young Ho

AU - Won, Ho Kim

AU - Moon, Changsuk

AU - Yun, Hwa Hong

AU - Eun, Su Yong

AU - Joo, Hyun Lim

AU - Joo, Sun Choi

AU - Song, Jihyun

AU - Jung, Myeong Ho

PY - 2005/11/18

Y1 - 2005/11/18

N2 - Neuronatin (Nnat) is selectively expressed in the neonatal brain and is involved in neuronal differentiation during brain development. However, Nnat also appears to be abundantly expressed in adipose tissue, and is conspicuously elevated in the adipose tissue of obese Zucker diabetic fatty rats compared with control lean Zucker lean control rats shown in our previous report. Here, we examined the expression of Nnat in adipose tissue and demonstrated that the ectopic expression of Nnat mediated by retroviral infection or stable transfection of 3T3-L1 pre-adipocytes stimulated differentiation into mature adipocytes with early induction of adipogenic transcription factors. Moreover, in 3T3-L1 cells overexpressing Nnat, increased intracellular free calcium levels and enhanced phosphorylation of cAMP-response element-binding protein (CREB) were observed, which appears to potentiate CCAAT/enhancer-binding protein (C/EBP)β, C/EBPδ, and C/EBPα transcriptional activities. Collectively, the data indicate that Nnat enhances CREB phosphorylation through increasing intracellular free calcium levels, which potentiates expression of adipogenic transcription factors resulting in heightened adipocyte differentiation. These findings contribute to a greater fundamental understanding of obesity, a clinically important risk factor in numerous diseases.

AB - Neuronatin (Nnat) is selectively expressed in the neonatal brain and is involved in neuronal differentiation during brain development. However, Nnat also appears to be abundantly expressed in adipose tissue, and is conspicuously elevated in the adipose tissue of obese Zucker diabetic fatty rats compared with control lean Zucker lean control rats shown in our previous report. Here, we examined the expression of Nnat in adipose tissue and demonstrated that the ectopic expression of Nnat mediated by retroviral infection or stable transfection of 3T3-L1 pre-adipocytes stimulated differentiation into mature adipocytes with early induction of adipogenic transcription factors. Moreover, in 3T3-L1 cells overexpressing Nnat, increased intracellular free calcium levels and enhanced phosphorylation of cAMP-response element-binding protein (CREB) were observed, which appears to potentiate CCAAT/enhancer-binding protein (C/EBP)β, C/EBPδ, and C/EBPα transcriptional activities. Collectively, the data indicate that Nnat enhances CREB phosphorylation through increasing intracellular free calcium levels, which potentiates expression of adipogenic transcription factors resulting in heightened adipocyte differentiation. These findings contribute to a greater fundamental understanding of obesity, a clinically important risk factor in numerous diseases.

KW - 3T3-L1

KW - Adipocyte differentiation

KW - CREB

KW - Intracellular calcium

KW - Neuronatin

UR - http://www.scopus.com/inward/record.url?scp=26444587461&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.09.078

DO - 10.1016/j.bbrc.2005.09.078

M3 - Article

C2 - 16223607

AN - SCOPUS:26444587461

VL - 337

SP - 481

EP - 489

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

SN - 0006-291X

IS - 2

ER -