Dynamic Adaptive Changes of the Ileum Transposed to the Proximal Small Intestine in Rats

Chang Ho Ahn, Sehyun Chae, Tae Jung Oh, Daehee Hwang, Young Min Cho

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Ileal transposition (IT) is an experimental surgery to investigate the role of the distal ileum in Roux-en-Y gastric bypass (RYGB) surgery. To systematically investigate the dynamic adaptation process of the ileum after IT, we performed transcriptome analyses of the transposed ileum compared with the ileum in situ at different postoperative time points. Methods: Sprague-Dawley rats fed a chow diet underwent IT or sham surgery. One and 4 weeks after IT or sham surgery, total RNA was extracted from the ileal tissue and subjected to transcriptome analyses using microarray. Results: Principal component analysis showed that the difference between weeks 1 and 4 was the largest, and the differences between the IT and sham groups were larger in week 4 than in week 1. We identified 1792 differentially expressed genes (DEGs) between IT and sham ileal tissues, including 659 and 1133 DEGs in weeks 1 and 4, respectively. Interestingly, only 45 and 24 DEGs were commonly up- or downregulated in weeks 1 and 4, indicating a marked transition during the adaptation process. Functional enrichment and network analyses showed that structural adaptation predominantly occurred in week 1, while metabolic and immune adaptations predominantly occurred in week 4. These analyses further revealed potential components that modulate structural adaptation (e.g., extracellular matrix) in week 1 and metabolic (e.g., glucose transporter) and immune (e.g., Th17 cells) adaptations in week 4. Conclusions: The transposed distal ileum underwent dynamic adaptation processes that may help explain the metabolic changes after RYGB.

Original languageEnglish
Pages (from-to)2399-2408
Number of pages10
JournalObesity Surgery
Volume29
Issue number8
DOIs
StatePublished - 15 Aug 2019

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Ileum
Small Intestine
Gastric Bypass
Gene Expression Profiling
Genes
Th17 Cells
Facilitative Glucose Transport Proteins
Principal Component Analysis
Extracellular Matrix
Sprague Dawley Rats
Down-Regulation
RNA
Diet

Keywords

  • Gut adaptation
  • Ileal transposition
  • Transcriptome analysis

Cite this

Ahn, Chang Ho ; Chae, Sehyun ; Oh, Tae Jung ; Hwang, Daehee ; Cho, Young Min. / Dynamic Adaptive Changes of the Ileum Transposed to the Proximal Small Intestine in Rats. In: Obesity Surgery. 2019 ; Vol. 29, No. 8. pp. 2399-2408.
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abstract = "Background: Ileal transposition (IT) is an experimental surgery to investigate the role of the distal ileum in Roux-en-Y gastric bypass (RYGB) surgery. To systematically investigate the dynamic adaptation process of the ileum after IT, we performed transcriptome analyses of the transposed ileum compared with the ileum in situ at different postoperative time points. Methods: Sprague-Dawley rats fed a chow diet underwent IT or sham surgery. One and 4 weeks after IT or sham surgery, total RNA was extracted from the ileal tissue and subjected to transcriptome analyses using microarray. Results: Principal component analysis showed that the difference between weeks 1 and 4 was the largest, and the differences between the IT and sham groups were larger in week 4 than in week 1. We identified 1792 differentially expressed genes (DEGs) between IT and sham ileal tissues, including 659 and 1133 DEGs in weeks 1 and 4, respectively. Interestingly, only 45 and 24 DEGs were commonly up- or downregulated in weeks 1 and 4, indicating a marked transition during the adaptation process. Functional enrichment and network analyses showed that structural adaptation predominantly occurred in week 1, while metabolic and immune adaptations predominantly occurred in week 4. These analyses further revealed potential components that modulate structural adaptation (e.g., extracellular matrix) in week 1 and metabolic (e.g., glucose transporter) and immune (e.g., Th17 cells) adaptations in week 4. Conclusions: The transposed distal ileum underwent dynamic adaptation processes that may help explain the metabolic changes after RYGB.",
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Dynamic Adaptive Changes of the Ileum Transposed to the Proximal Small Intestine in Rats. / Ahn, Chang Ho; Chae, Sehyun; Oh, Tae Jung; Hwang, Daehee; Cho, Young Min.

In: Obesity Surgery, Vol. 29, No. 8, 15.08.2019, p. 2399-2408.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Dynamic Adaptive Changes of the Ileum Transposed to the Proximal Small Intestine in Rats

AU - Ahn, Chang Ho

AU - Chae, Sehyun

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AU - Hwang, Daehee

AU - Cho, Young Min

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N2 - Background: Ileal transposition (IT) is an experimental surgery to investigate the role of the distal ileum in Roux-en-Y gastric bypass (RYGB) surgery. To systematically investigate the dynamic adaptation process of the ileum after IT, we performed transcriptome analyses of the transposed ileum compared with the ileum in situ at different postoperative time points. Methods: Sprague-Dawley rats fed a chow diet underwent IT or sham surgery. One and 4 weeks after IT or sham surgery, total RNA was extracted from the ileal tissue and subjected to transcriptome analyses using microarray. Results: Principal component analysis showed that the difference between weeks 1 and 4 was the largest, and the differences between the IT and sham groups were larger in week 4 than in week 1. We identified 1792 differentially expressed genes (DEGs) between IT and sham ileal tissues, including 659 and 1133 DEGs in weeks 1 and 4, respectively. Interestingly, only 45 and 24 DEGs were commonly up- or downregulated in weeks 1 and 4, indicating a marked transition during the adaptation process. Functional enrichment and network analyses showed that structural adaptation predominantly occurred in week 1, while metabolic and immune adaptations predominantly occurred in week 4. These analyses further revealed potential components that modulate structural adaptation (e.g., extracellular matrix) in week 1 and metabolic (e.g., glucose transporter) and immune (e.g., Th17 cells) adaptations in week 4. Conclusions: The transposed distal ileum underwent dynamic adaptation processes that may help explain the metabolic changes after RYGB.

AB - Background: Ileal transposition (IT) is an experimental surgery to investigate the role of the distal ileum in Roux-en-Y gastric bypass (RYGB) surgery. To systematically investigate the dynamic adaptation process of the ileum after IT, we performed transcriptome analyses of the transposed ileum compared with the ileum in situ at different postoperative time points. Methods: Sprague-Dawley rats fed a chow diet underwent IT or sham surgery. One and 4 weeks after IT or sham surgery, total RNA was extracted from the ileal tissue and subjected to transcriptome analyses using microarray. Results: Principal component analysis showed that the difference between weeks 1 and 4 was the largest, and the differences between the IT and sham groups were larger in week 4 than in week 1. We identified 1792 differentially expressed genes (DEGs) between IT and sham ileal tissues, including 659 and 1133 DEGs in weeks 1 and 4, respectively. Interestingly, only 45 and 24 DEGs were commonly up- or downregulated in weeks 1 and 4, indicating a marked transition during the adaptation process. Functional enrichment and network analyses showed that structural adaptation predominantly occurred in week 1, while metabolic and immune adaptations predominantly occurred in week 4. These analyses further revealed potential components that modulate structural adaptation (e.g., extracellular matrix) in week 1 and metabolic (e.g., glucose transporter) and immune (e.g., Th17 cells) adaptations in week 4. Conclusions: The transposed distal ileum underwent dynamic adaptation processes that may help explain the metabolic changes after RYGB.

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