DOPA-decarboxylation in the striata of rats with unilateral substantia nigra lesions

Un Jung Kang, Dong H. Park, Thomas Wessel, Harriet Baker, Tong H. Joh

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

The source and site of the DOPA decarboxylation to dopamine in Parkinson's disease (PD) and animal models of PD are controversial. Since most of aromatic l-amino acid decarboxylase (AADC) are lost along with the degenerating dopaminergic neurons, we addressed the possibility that other decarboxylases or a novel protein that is structurally different from AADC decarboxylate l-DOPA in the denervated striatum. Immunotitration of the extracts from the denervated striatum with AADC antibody showed that all activity can be attributed to AADC-immunoreactive protein. We then investigated if there are non-dopaminergic intrinsic striatal neurons that express AADC. No evidence of such neurons was noted by immunocytochemistry and in situ hybridization.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalNeuroscience Letters
Volume147
Issue number1
DOIs
StatePublished - 23 Nov 1992

Fingerprint

Decarboxylation
Carboxy-Lyases
Substantia Nigra
Amino Acids
Parkinson Disease
Aromatic-L-Amino-Acid Decarboxylases
Neurons
Corpus Striatum
Dopaminergic Neurons
In Situ Hybridization
Dopamine
Proteins
Animal Models
Immunohistochemistry
Antibodies

Keywords

  • 6-Hydroxydopamine
  • Aromatic l-amino acid decarboxylase
  • DOPA decarboxylase
  • Parkinson's disease
  • Striatum
  • l-DOPA

Cite this

Kang, Un Jung ; Park, Dong H. ; Wessel, Thomas ; Baker, Harriet ; Joh, Tong H. / DOPA-decarboxylation in the striata of rats with unilateral substantia nigra lesions. In: Neuroscience Letters. 1992 ; Vol. 147, No. 1. pp. 53-57.
@article{c7026ee399b64f308381303fe94259ba,
title = "DOPA-decarboxylation in the striata of rats with unilateral substantia nigra lesions",
abstract = "The source and site of the DOPA decarboxylation to dopamine in Parkinson's disease (PD) and animal models of PD are controversial. Since most of aromatic l-amino acid decarboxylase (AADC) are lost along with the degenerating dopaminergic neurons, we addressed the possibility that other decarboxylases or a novel protein that is structurally different from AADC decarboxylate l-DOPA in the denervated striatum. Immunotitration of the extracts from the denervated striatum with AADC antibody showed that all activity can be attributed to AADC-immunoreactive protein. We then investigated if there are non-dopaminergic intrinsic striatal neurons that express AADC. No evidence of such neurons was noted by immunocytochemistry and in situ hybridization.",
keywords = "6-Hydroxydopamine, Aromatic l-amino acid decarboxylase, DOPA decarboxylase, Parkinson's disease, Striatum, l-DOPA",
author = "Kang, {Un Jung} and Park, {Dong H.} and Thomas Wessel and Harriet Baker and Joh, {Tong H.}",
year = "1992",
month = "11",
day = "23",
doi = "10.1016/0304-3940(92)90773-Z",
language = "English",
volume = "147",
pages = "53--57",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

DOPA-decarboxylation in the striata of rats with unilateral substantia nigra lesions. / Kang, Un Jung; Park, Dong H.; Wessel, Thomas; Baker, Harriet; Joh, Tong H.

In: Neuroscience Letters, Vol. 147, No. 1, 23.11.1992, p. 53-57.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - DOPA-decarboxylation in the striata of rats with unilateral substantia nigra lesions

AU - Kang, Un Jung

AU - Park, Dong H.

AU - Wessel, Thomas

AU - Baker, Harriet

AU - Joh, Tong H.

PY - 1992/11/23

Y1 - 1992/11/23

N2 - The source and site of the DOPA decarboxylation to dopamine in Parkinson's disease (PD) and animal models of PD are controversial. Since most of aromatic l-amino acid decarboxylase (AADC) are lost along with the degenerating dopaminergic neurons, we addressed the possibility that other decarboxylases or a novel protein that is structurally different from AADC decarboxylate l-DOPA in the denervated striatum. Immunotitration of the extracts from the denervated striatum with AADC antibody showed that all activity can be attributed to AADC-immunoreactive protein. We then investigated if there are non-dopaminergic intrinsic striatal neurons that express AADC. No evidence of such neurons was noted by immunocytochemistry and in situ hybridization.

AB - The source and site of the DOPA decarboxylation to dopamine in Parkinson's disease (PD) and animal models of PD are controversial. Since most of aromatic l-amino acid decarboxylase (AADC) are lost along with the degenerating dopaminergic neurons, we addressed the possibility that other decarboxylases or a novel protein that is structurally different from AADC decarboxylate l-DOPA in the denervated striatum. Immunotitration of the extracts from the denervated striatum with AADC antibody showed that all activity can be attributed to AADC-immunoreactive protein. We then investigated if there are non-dopaminergic intrinsic striatal neurons that express AADC. No evidence of such neurons was noted by immunocytochemistry and in situ hybridization.

KW - 6-Hydroxydopamine

KW - Aromatic l-amino acid decarboxylase

KW - DOPA decarboxylase

KW - Parkinson's disease

KW - Striatum

KW - l-DOPA

UR - http://www.scopus.com/inward/record.url?scp=0026483508&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(92)90773-Z

DO - 10.1016/0304-3940(92)90773-Z

M3 - Article

VL - 147

SP - 53

EP - 57

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -