Direct effect of chenodeoxycholic acid on differentiation of mouse embryonic stem cells cultured under feeder-free culture conditions

Soon Jung Park, Seul Bi Lee, Dong-Sup Lee, Young Joon Ryu, Gene Lee, Jaejin Cho

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)

Abstract

Chenodeoxycholic acid (CDCA), a farnesoid X receptor (FXR) ligand, is a member of the nuclear receptor family and is probably involved in regulating the cellular activities of embryonic stem (ES) cells. Recently, although it was reported that the FXR ligand can mediate differentiation, apoptosis, and/or growth arrest in several cell types, it is still not well known how CDCA mediates effects in ES cells. Therefore, we investigated the direct effect of CDCA on mES cells. Feeder-free mES cells were treated in a dose-dependent manner with CDCA (50, 100, and 200 M) for 72 h, and then a 100 M CDCA treatment was performed for an additional 72 h. We analyzed the morphology, cell growth, cell characteristics, immunocytochemistry, and RT-PCR. In CDCA-treated cells, we observed the disappearance of pluripotent stem cell markers including alkaline phosphatase, Oct4, and Nanog and a time- and dose-dependent increase in expression of nestin, PAX6, and α-smooth muscle actin, but not α-fetoprotein. The 100 M CDCA-treated cells in their second passage continued this differentiation pattern similar to those in the controls. In conclusion, these results suggest that CDCA can guide mES cells by an FXR-independent pathway to differentiate into ectoderm and/or mesoderm, but not endoderm.

Original languageEnglish
Article number375076
JournalBioMed Research International
Volume2013
DOIs
StatePublished - 11 Mar 2013

Fingerprint

Chenodeoxycholic Acid
Stem cells
Cell culture
Embryonic Stem Cells
Cells
Fetal Proteins
Ligands
Nestin
Pluripotent Stem Cells
Endoderm
Ectoderm
Mouse Embryonic Stem Cells
Cell growth
Mesoderm
Cytoplasmic and Nuclear Receptors
Growth
Nuclear Family
Smooth Muscle
Alkaline Phosphatase
Muscle

Cite this

@article{da3a239253fb40bbac41326c7faccd3f,
title = "Direct effect of chenodeoxycholic acid on differentiation of mouse embryonic stem cells cultured under feeder-free culture conditions",
abstract = "Chenodeoxycholic acid (CDCA), a farnesoid X receptor (FXR) ligand, is a member of the nuclear receptor family and is probably involved in regulating the cellular activities of embryonic stem (ES) cells. Recently, although it was reported that the FXR ligand can mediate differentiation, apoptosis, and/or growth arrest in several cell types, it is still not well known how CDCA mediates effects in ES cells. Therefore, we investigated the direct effect of CDCA on mES cells. Feeder-free mES cells were treated in a dose-dependent manner with CDCA (50, 100, and 200 M) for 72 h, and then a 100 M CDCA treatment was performed for an additional 72 h. We analyzed the morphology, cell growth, cell characteristics, immunocytochemistry, and RT-PCR. In CDCA-treated cells, we observed the disappearance of pluripotent stem cell markers including alkaline phosphatase, Oct4, and Nanog and a time- and dose-dependent increase in expression of nestin, PAX6, and α-smooth muscle actin, but not α-fetoprotein. The 100 M CDCA-treated cells in their second passage continued this differentiation pattern similar to those in the controls. In conclusion, these results suggest that CDCA can guide mES cells by an FXR-independent pathway to differentiate into ectoderm and/or mesoderm, but not endoderm.",
author = "Park, {Soon Jung} and Lee, {Seul Bi} and Dong-Sup Lee and Ryu, {Young Joon} and Gene Lee and Jaejin Cho",
year = "2013",
month = "3",
day = "11",
doi = "10.1155/2013/375076",
language = "English",
volume = "2013",
journal = "BioMed research international",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

Direct effect of chenodeoxycholic acid on differentiation of mouse embryonic stem cells cultured under feeder-free culture conditions. / Park, Soon Jung; Lee, Seul Bi; Lee, Dong-Sup; Ryu, Young Joon; Lee, Gene; Cho, Jaejin.

In: BioMed Research International, Vol. 2013, 375076, 11.03.2013.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Direct effect of chenodeoxycholic acid on differentiation of mouse embryonic stem cells cultured under feeder-free culture conditions

AU - Park, Soon Jung

AU - Lee, Seul Bi

AU - Lee, Dong-Sup

AU - Ryu, Young Joon

AU - Lee, Gene

AU - Cho, Jaejin

PY - 2013/3/11

Y1 - 2013/3/11

N2 - Chenodeoxycholic acid (CDCA), a farnesoid X receptor (FXR) ligand, is a member of the nuclear receptor family and is probably involved in regulating the cellular activities of embryonic stem (ES) cells. Recently, although it was reported that the FXR ligand can mediate differentiation, apoptosis, and/or growth arrest in several cell types, it is still not well known how CDCA mediates effects in ES cells. Therefore, we investigated the direct effect of CDCA on mES cells. Feeder-free mES cells were treated in a dose-dependent manner with CDCA (50, 100, and 200 M) for 72 h, and then a 100 M CDCA treatment was performed for an additional 72 h. We analyzed the morphology, cell growth, cell characteristics, immunocytochemistry, and RT-PCR. In CDCA-treated cells, we observed the disappearance of pluripotent stem cell markers including alkaline phosphatase, Oct4, and Nanog and a time- and dose-dependent increase in expression of nestin, PAX6, and α-smooth muscle actin, but not α-fetoprotein. The 100 M CDCA-treated cells in their second passage continued this differentiation pattern similar to those in the controls. In conclusion, these results suggest that CDCA can guide mES cells by an FXR-independent pathway to differentiate into ectoderm and/or mesoderm, but not endoderm.

AB - Chenodeoxycholic acid (CDCA), a farnesoid X receptor (FXR) ligand, is a member of the nuclear receptor family and is probably involved in regulating the cellular activities of embryonic stem (ES) cells. Recently, although it was reported that the FXR ligand can mediate differentiation, apoptosis, and/or growth arrest in several cell types, it is still not well known how CDCA mediates effects in ES cells. Therefore, we investigated the direct effect of CDCA on mES cells. Feeder-free mES cells were treated in a dose-dependent manner with CDCA (50, 100, and 200 M) for 72 h, and then a 100 M CDCA treatment was performed for an additional 72 h. We analyzed the morphology, cell growth, cell characteristics, immunocytochemistry, and RT-PCR. In CDCA-treated cells, we observed the disappearance of pluripotent stem cell markers including alkaline phosphatase, Oct4, and Nanog and a time- and dose-dependent increase in expression of nestin, PAX6, and α-smooth muscle actin, but not α-fetoprotein. The 100 M CDCA-treated cells in their second passage continued this differentiation pattern similar to those in the controls. In conclusion, these results suggest that CDCA can guide mES cells by an FXR-independent pathway to differentiate into ectoderm and/or mesoderm, but not endoderm.

UR - http://www.scopus.com/inward/record.url?scp=84874610544&partnerID=8YFLogxK

U2 - 10.1155/2013/375076

DO - 10.1155/2013/375076

M3 - Article

VL - 2013

JO - BioMed research international

JF - BioMed research international

SN - 2314-6133

M1 - 375076

ER -