Differentiation of Borrelia burgdorferi sensu lato through groEL gene analysis

Seung Hyun Lee, Jung Hee Lee, Hyo Soon Park, Won Jong Jang, Seong Eun Koh, Young Mok Yang, Bum Joon Kim, Yoon Hoh Kook, Kyung Hee Park

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The nucleotide sequences (310 bp) of the groEL gene, which encode the 60-kDa heat shock protein GroEL from 31 reference strains of Borrelia were determined and compared. More than 92.3% similarity was observed among Borrelia burgdorferi sensu lato strains. In the phylogenetic tree constructed with the maximum-likelihood method, each species of B. burgdorferi sensu lato was differentiated as a distinct entity. We developed polymerase chain reaction-restriction fragment length polymorphism analysis using a specific single amino acid variation [N213 (AAT)→S (AGC or AGT)] between B. burgdorferi sensu stricto strains and the other B. burgdorferi sensu lato strains. These results showed that the groEL gene is useful for differentiation of B. burgdorferi sensu lato.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalFEMS Microbiology Letters
Volume222
Issue number1
DOIs
StatePublished - 16 May 2003

Keywords

  • B. burgdorferi sensu lato
  • Differentiation
  • GroEL gene

Cite this

Lee, S. H., Lee, J. H., Park, H. S., Jang, W. J., Koh, S. E., Yang, Y. M., Kim, B. J., Kook, Y. H., & Park, K. H. (2003). Differentiation of Borrelia burgdorferi sensu lato through groEL gene analysis. FEMS Microbiology Letters, 222(1), 51-57. https://doi.org/10.1016/S0378-1097(03)00237-4