Differentiation Between Hepatocellular Carcinoma and Colorectal Cancer Liver Metastases on High-Resolution Magic Angle Spinning Spectroscopy: Preliminary Study

So Yeon Kim, Siwon Kim, Chul Woong Woo, Jae Ho Byun, Seung Soo Lee, Moon Gyu Lee, Haeryoung Kim, Kyoungho Lee, Young Hoon Kim, Jai Young Cho, Suhkmann Kim, Jin Seong Lee

Research output: Contribution to journalArticle

Abstract

To explore the potential of high-resolution magic angle spinning (HRMAS) 1H nuclear magnetic resonance (NMR) spectroscopy for differentiation and metabolite characterization of hepatocellular carcinoma (HCC) and colorectal liver metastases (CRLM), we prospectively included 21 pathologically confirmed malignant hepatic tumors (8 HCC and 13 CRLM) and 26 non-tumorous hepatic parenchyma from 26 patients who underwent hepatic tumor resection. Using intact tissue samples obtained during surgery, HRMAS 1H NMR spectroscopy was performed at 11.7 T. All observable metabolite signals were acquired using a water-presaturated standard one-dimensional Carr-Purcell-Meiboom-Gill sequence. Metabolomic profiles contributing to the differentiation of HCC and CRLM and of each tumor and non-tumorous hepatic parenchyma were represented by orthogonal partial least squares discriminant analysis (OPLS-DA) and loading plots. Metabolite intensity normalized by total spectral intensities in both tumors was compared using student's t tests. OPLS-DA and loading plots demonstrated good separation between tumors and non-tumorous hepatic parenchyma. The metabolomic characteristics of HCC showed separation from those of CRLMs according to OPLS-DA. Compared with CRLM, HCC showed significantly elevated levels of glucose (P < 0.01) and sn-Glycero-3-phosphocholine (P < 0.01), and decreased levels of hypoxanthine (P = 0.04). HCC and CRLM could be differentiated by the metabolic profile using HRMAS 1H NMR spectroscopy.

Original languageEnglish
Pages (from-to)19-35
Number of pages17
JournalApplied Magnetic Resonance
Volume45
Issue number1
DOIs
StatePublished - 1 Jan 2014

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metastasis
liver
metal spinning
cancer
tumors
metabolites
high resolution
magnetic resonance spectroscopy
spectroscopy
nuclear magnetic resonance
plots
profiles
glucose
surgery
students
water

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title = "Differentiation Between Hepatocellular Carcinoma and Colorectal Cancer Liver Metastases on High-Resolution Magic Angle Spinning Spectroscopy: Preliminary Study",
abstract = "To explore the potential of high-resolution magic angle spinning (HRMAS) 1H nuclear magnetic resonance (NMR) spectroscopy for differentiation and metabolite characterization of hepatocellular carcinoma (HCC) and colorectal liver metastases (CRLM), we prospectively included 21 pathologically confirmed malignant hepatic tumors (8 HCC and 13 CRLM) and 26 non-tumorous hepatic parenchyma from 26 patients who underwent hepatic tumor resection. Using intact tissue samples obtained during surgery, HRMAS 1H NMR spectroscopy was performed at 11.7 T. All observable metabolite signals were acquired using a water-presaturated standard one-dimensional Carr-Purcell-Meiboom-Gill sequence. Metabolomic profiles contributing to the differentiation of HCC and CRLM and of each tumor and non-tumorous hepatic parenchyma were represented by orthogonal partial least squares discriminant analysis (OPLS-DA) and loading plots. Metabolite intensity normalized by total spectral intensities in both tumors was compared using student's t tests. OPLS-DA and loading plots demonstrated good separation between tumors and non-tumorous hepatic parenchyma. The metabolomic characteristics of HCC showed separation from those of CRLMs according to OPLS-DA. Compared with CRLM, HCC showed significantly elevated levels of glucose (P < 0.01) and sn-Glycero-3-phosphocholine (P < 0.01), and decreased levels of hypoxanthine (P = 0.04). HCC and CRLM could be differentiated by the metabolic profile using HRMAS 1H NMR spectroscopy.",
author = "Kim, {So Yeon} and Siwon Kim and Woo, {Chul Woong} and Byun, {Jae Ho} and Lee, {Seung Soo} and Lee, {Moon Gyu} and Haeryoung Kim and Kyoungho Lee and Kim, {Young Hoon} and Cho, {Jai Young} and Suhkmann Kim and Lee, {Jin Seong}",
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Differentiation Between Hepatocellular Carcinoma and Colorectal Cancer Liver Metastases on High-Resolution Magic Angle Spinning Spectroscopy : Preliminary Study. / Kim, So Yeon; Kim, Siwon; Woo, Chul Woong; Byun, Jae Ho; Lee, Seung Soo; Lee, Moon Gyu; Kim, Haeryoung; Lee, Kyoungho; Kim, Young Hoon; Cho, Jai Young; Kim, Suhkmann; Lee, Jin Seong.

In: Applied Magnetic Resonance, Vol. 45, No. 1, 01.01.2014, p. 19-35.

Research output: Contribution to journalArticle

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T1 - Differentiation Between Hepatocellular Carcinoma and Colorectal Cancer Liver Metastases on High-Resolution Magic Angle Spinning Spectroscopy

T2 - Preliminary Study

AU - Kim, So Yeon

AU - Kim, Siwon

AU - Woo, Chul Woong

AU - Byun, Jae Ho

AU - Lee, Seung Soo

AU - Lee, Moon Gyu

AU - Kim, Haeryoung

AU - Lee, Kyoungho

AU - Kim, Young Hoon

AU - Cho, Jai Young

AU - Kim, Suhkmann

AU - Lee, Jin Seong

PY - 2014/1/1

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