Differentially up-regulated genes in proliferating porcine neonatal pancreas cells caused by epidermal growth factor

Sung Yoon Jeon, Kwang Hyun Baek, Yong Soo Kim, Chung Gyu Park, Hyuk Sang Kwon, Seung Hyun Ko, Ki Ho Song, Soon Jib Yoo, Hyun Shik Son, Bong Yun Cha, Kwang Woo Lee, Ho Young Son, Sung Koo Kang, Kun Ho Yoon

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10 Scopus citations


Pancreatic duct cells are considered to be a major source for β-cell regeneration or neogenesis. Although epidermal growth factor (EGF) is a well-known important growth factor for pancreas development, the control of pancreatic duct cell growth and differentiation by EGF is poorly understood. In this study, we focused on identifying the genes that were differentially up-regulated in response to EGF stimulation using monolayer cultured porcine neonatal pancreas cells. Cells were obtained from 1 to 3 day old pigs, dispersed and cultured for 8 days. Monolayer cultured porcine pancreas cells were comprised of duct cells and some endocrine and mesenchymal cells (75.2 ± 15.1, 19.6 ± 4.9, and 9.5 ± 3.1%, respectively). After 16 h in serum free media, cells were treated with 100 μg/L EGF for 24 h. Differentially expressed genes were screened by subtractive hybridization. 3H-thymidine uptake was significantly increased by EGF with time (untreated vs. 24 h treated, untreated vs. 48 h treated: 305.5 ± 3.5 cpm vs. 380.3 ± 17.3 cpm (P < 0.05), 309.2 ± 4.51 vs. 929 ± 9.19 cpm, (P < 0.005), respectively). Three hundred and fify cDNA clones were obtained by subtractive hybridization and the inserts were confirmed in 161 colonies and then sequenced. Finally, we found increased mRNA expression of five unknown and five known genes, including cytochrome c oxidase subunit I (COI), cyclooxygenase-2 (COX-2), matrix metalloproteinase-13 (MMP-13), Wiskott-Aldrich syndrome protein interacting protein (WASPIP), and hyaluronan synthase-2 (HAS-2). We confirmed the up-regulation of these genes by Northern blot and semi-quantitative RT-PCR at various time points. The present findings opened new targets for the research on the mechanisms of pancreatic duct cell proliferation by EGF.

Original languageEnglish
Pages (from-to)354-364
Number of pages11
JournalJournal of Cellular Biochemistry
Issue number2
StatePublished - 1 Dec 2004


  • Epidermal growth factor
  • Matrix metalloproteinase-13
  • Pancreas duct cell
  • Pig
  • Subtractive hybridization

Cite this

Jeon, S. Y., Baek, K. H., Kim, Y. S., Park, C. G., Kwon, H. S., Ko, S. H., Song, K. H., Yoo, S. J., Son, H. S., Cha, B. Y., Lee, K. W., Son, H. Y., Kang, S. K., & Yoon, K. H. (2004). Differentially up-regulated genes in proliferating porcine neonatal pancreas cells caused by epidermal growth factor. Journal of Cellular Biochemistry, 91(2), 354-364. https://doi.org/10.1002/jcb.10752