Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants

Jong Hyeok Jung, Hyun Jung Hong, Aziz Gharderpour, Jae Young Cho, Bum Seo Baek, Yong Hur, Byoung Choul Kim, Donghyun Kim, Seung Yong Seong, Jae Young Lim, Sang Uk Seo

Research output: Contribution to journalArticle

Abstract

Urinary tract infection (UTI) is one of the most common bacterial infections in infants less than age 1 year. UTIs frequently recur and result in long-term effects include sepsis and renal scarring. Uropathogenic Escherichia coli (UPEC), the most prevalent organism found in UTIs, can cause host inflammation via various virulence factors including hemolysin and cytotoxic necrotizing factors by inducing inflammatory cytokines such as interleukin (IL)-1β. However, the ability of each UPEC organism to induce IL-1β production may differ by strain. Furthermore, the correlation between differential IL-1β induction and its relevance in pathology has not been well studied. In this study, we isolated UPEC from children under age 24 months and infected bone-marrow derived macrophages with the isolates to investigate secretion of IL-1β. We found that children with higher concentrations of C-reactive protein (CRP) were more likely to harbor phylotype B2 UPEC strains that induced more IL-1β production than phylotype D. We also observed a significant correlation between serum CRP level and in vitro IL-1β induction by phylotype B2 UPEC bacteria. Our results highlight the diversity of UPEC in terms of IL-1β induction capacity in macrophages and suggest a potential pathogenic role in UTIs by inducing inflammation in infants.

Original languageEnglish
Article number15654
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - 1 Dec 2019

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Uropathogenic Escherichia coli
Interleukin-1
C-Reactive Protein
Blood Proteins
Macrophages
Inflammation
Hemolysin Proteins
Virulence Factors
Bacterial Infections
Urinary Tract Infections
Cicatrix
Sepsis
Pathology
Cytokines
Bacteria
Kidney

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Jung, Jong Hyeok ; Hong, Hyun Jung ; Gharderpour, Aziz ; Cho, Jae Young ; Baek, Bum Seo ; Hur, Yong ; Kim, Byoung Choul ; Kim, Donghyun ; Seong, Seung Yong ; Lim, Jae Young ; Seo, Sang Uk. / Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Urinary tract infection (UTI) is one of the most common bacterial infections in infants less than age 1 year. UTIs frequently recur and result in long-term effects include sepsis and renal scarring. Uropathogenic Escherichia coli (UPEC), the most prevalent organism found in UTIs, can cause host inflammation via various virulence factors including hemolysin and cytotoxic necrotizing factors by inducing inflammatory cytokines such as interleukin (IL)-1β. However, the ability of each UPEC organism to induce IL-1β production may differ by strain. Furthermore, the correlation between differential IL-1β induction and its relevance in pathology has not been well studied. In this study, we isolated UPEC from children under age 24 months and infected bone-marrow derived macrophages with the isolates to investigate secretion of IL-1β. We found that children with higher concentrations of C-reactive protein (CRP) were more likely to harbor phylotype B2 UPEC strains that induced more IL-1β production than phylotype D. We also observed a significant correlation between serum CRP level and in vitro IL-1β induction by phylotype B2 UPEC bacteria. Our results highlight the diversity of UPEC in terms of IL-1β induction capacity in macrophages and suggest a potential pathogenic role in UTIs by inducing inflammation in infants.",
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Differential interleukin-1β induction by uropathogenic Escherichia coli correlates with its phylotype and serum C-reactive protein levels in Korean infants. / Jung, Jong Hyeok; Hong, Hyun Jung; Gharderpour, Aziz; Cho, Jae Young; Baek, Bum Seo; Hur, Yong; Kim, Byoung Choul; Kim, Donghyun; Seong, Seung Yong; Lim, Jae Young; Seo, Sang Uk.

In: Scientific Reports, Vol. 9, No. 1, 15654, 01.12.2019.

Research output: Contribution to journalArticle

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AU - Hur, Yong

AU - Kim, Byoung Choul

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AU - Seo, Sang Uk

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