Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals

Jin Jae Lee, Sae Hoon Kim, Min Jung Lee, Byung Keun Kim, Woo Jung Song, Heung Woo Park, Sang Heon Cho, Soo Jong Hong, Yoon Seok Chang, Bong Soo Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Microbes in the airway have been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma. Methods: Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing. Results: The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV 1% predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis—which may all be related to increased inflammation and colonization of pathogenic bacteria—were higher in young adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity. Conclusions: These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.

Original languageEnglish
Pages (from-to)709-719
Number of pages11
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume74
Issue number4
DOIs
StatePublished - 1 Apr 2019

Fingerprint

Microbiota
Young Adult
Asthma
Genes
Age Groups
Caprolactam
Dysbiosis
Metagenome
Inflammation
Pentose Phosphate Pathway
Air Pollutants
Peroxisome Proliferator-Activated Receptors
Flagella
Lysine
Polysaccharides
Lipopolysaccharides
Morbidity
Bacteria

Keywords

  • adult
  • asthma
  • elderly
  • microbiome
  • upper airway

Cite this

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title = "Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals",
abstract = "Background: Microbes in the airway have been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma. Methods: Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing. Results: The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV 1{\%} predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis—which may all be related to increased inflammation and colonization of pathogenic bacteria—were higher in young adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity. Conclusions: These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.",
keywords = "adult, asthma, elderly, microbiome, upper airway",
author = "Lee, {Jin Jae} and Kim, {Sae Hoon} and Lee, {Min Jung} and Kim, {Byung Keun} and Song, {Woo Jung} and Park, {Heung Woo} and Cho, {Sang Heon} and Hong, {Soo Jong} and Chang, {Yoon Seok} and Kim, {Bong Soo}",
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Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals. / Lee, Jin Jae; Kim, Sae Hoon; Lee, Min Jung; Kim, Byung Keun; Song, Woo Jung; Park, Heung Woo; Cho, Sang Heon; Hong, Soo Jong; Chang, Yoon Seok; Kim, Bong Soo.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 74, No. 4, 01.04.2019, p. 709-719.

Research output: Contribution to journalArticle

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T1 - Different upper airway microbiome and their functional genes associated with asthma in young adults and elderly individuals

AU - Lee, Jin Jae

AU - Kim, Sae Hoon

AU - Lee, Min Jung

AU - Kim, Byung Keun

AU - Song, Woo Jung

AU - Park, Heung Woo

AU - Cho, Sang Heon

AU - Hong, Soo Jong

AU - Chang, Yoon Seok

AU - Kim, Bong Soo

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Background: Microbes in the airway have been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma. Methods: Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing. Results: The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV 1% predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis—which may all be related to increased inflammation and colonization of pathogenic bacteria—were higher in young adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity. Conclusions: These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.

AB - Background: Microbes in the airway have been shown to be associated with the pathogenesis of asthma. The upper airway microbiome influences the dysbiosis of the lower airway microbiome. However, to date, the influence of upper airway microbiome for adult and elderly asthma has not been fully elucidated. Here, the metagenome of upper airway microbiome of young adults and elderly was analyzed to identify their association with adult asthma. Methods: Nasopharyngeal swabs were collected from young adult and elderly asthma patients and non-asthmatic subjects. The compositions and functional genes of airway microbiome were analyzed by high-throughput sequencing. Results: The composition of microbiota differed between young adult and elderly, and it was different between asthmatics and non-asthmatics in each age group. Different bacteria were related to FEV 1% predicted in each age group. Genes related to lysine degradation, N-glycan biosynthesis, caprolactam degradation, and PPAR signaling pathway, which could be related to the reduction in inflammation and degradation of air pollutants, were higher in non-asthmatics. Genes related to pentose phosphate pathway, lipopolysaccharide biosynthesis, flagella assembly, and bacterial chemotaxis—which may all be related to increased inflammation and colonization of pathogenic bacteria—were higher in young adult asthmatic patients. However, the functional genes of airway microbiome in elderly patients were not significantly different according to asthma morbidity. Conclusions: These results suggest that the composition and function of upper airway microbiome could influence asthma pathogenesis, and the microbiome could play various roles depending on the age group.

KW - adult

KW - asthma

KW - elderly

KW - microbiome

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