Development of a peptide aptamer pair-linked rapid fluorescent diagnostic system for Zika virus detection

Anh Thi Viet Nguyen, Bao Tuan Duong, Hyun Park, Seon Ju Yeo

Research output: Contribution to journalArticlepeer-review

Abstract

A rapid diagnostic system employing an antigen detection biosensing method is needed to discriminate between Zika virus (ZIKV) and Dengue virus (DENV) due to their close antigenic homology. We developed a novel peptide pair-based flow immunochromatographic test strip (FICT) assay to detect ZIKV. Peptide aptamers, P6.1 (KQERNNWPLTWT), P29.1 (KYTTSTLKSGV), and B2.33 (KRHVWVSLSYSCAEA) were designed by paratopes and modified against the ZIKV envelope protein based on the binding affinity. An antibody-free lateral FICT was developed using a pair of peptide aptamers. In the rapid diagnostic strip, the limit of detection (LOD) for the B2.33-P6.1 peptide pair for ZIKV was 2 × 104 tissue culture infective dose TCID50/mL. Significantly, FICT could discriminate ZIKV from DENV. The stability and performance of FICT were confirmed using human sera and urine, showing a comparable LOD value. Our study demonstrated that in silico modeling could be used to develop a novel peptide pair-based FICT assay for detecting ZIKV by a rapid diagnostic test.

Original languageEnglish
Article number113768
JournalBiosensors and Bioelectronics
Volume197
DOIs
StatePublished - 1 Feb 2022

Keywords

  • Antibody-free peptide pair-linked rapid diagnostic system
  • Biosensor
  • Peptide aptamer
  • Zika virus

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