Development of a multiplex PCR system for the simultaneous detection of the shrimp species Fenneropenaeus chinensis, Litopenaeus vannamei, and Penaeus monodon

You Won Lee, Seung Ha Lee, Chun Feng Xin, Ji Hun Shin, Eunhee Shin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Due to mixed use of shrimp in foodstuffs, it is important to distinguish Fenneropenaeus chinensis from other shrimp species, such as Litopenaeus vannamei and Penaeus monodon. For this purpose, we have developed a multiplex PCR method to detect the simultaneous presence of all three species. Universal primers specific to the 16S ribosomal RNA subunit were used to sequence and ascertain the species in the samples. In this study, specific primers were designed to result in a unique PCR product size (143, 260, and 348 bp for L. vannamei, F. chinensis and P. monodon, respectively). In addition, duplex and triplex PCR protocols were developed to concurrently and rapidly detect these three shrimp species without nonspecific gene amplification. This multiplex PCR system is expected to be widely used to distinguish shrimp species that should be monitored to prevent their mixed use in food.

Original languageEnglish
Pages (from-to)104-108
Number of pages5
JournalJournal of AOAC International
Volume100
Issue number1
DOIs
StatePublished - 1 Jan 2017

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16S Ribosomal RNA
Fenneropenaeus chinensis
Penaeidae
Penaeus monodon
Multiplex Polymerase Chain Reaction
Litopenaeus vannamei
shrimp
Ribosome Subunits
Gene Amplification
duplex
Food
Polymerase Chain Reaction
polymerase chain reaction
ribosomal RNA
RNA
amplification
detection
food
gene
sampling

Cite this

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title = "Development of a multiplex PCR system for the simultaneous detection of the shrimp species Fenneropenaeus chinensis, Litopenaeus vannamei, and Penaeus monodon",
abstract = "Due to mixed use of shrimp in foodstuffs, it is important to distinguish Fenneropenaeus chinensis from other shrimp species, such as Litopenaeus vannamei and Penaeus monodon. For this purpose, we have developed a multiplex PCR method to detect the simultaneous presence of all three species. Universal primers specific to the 16S ribosomal RNA subunit were used to sequence and ascertain the species in the samples. In this study, specific primers were designed to result in a unique PCR product size (143, 260, and 348 bp for L. vannamei, F. chinensis and P. monodon, respectively). In addition, duplex and triplex PCR protocols were developed to concurrently and rapidly detect these three shrimp species without nonspecific gene amplification. This multiplex PCR system is expected to be widely used to distinguish shrimp species that should be monitored to prevent their mixed use in food.",
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Development of a multiplex PCR system for the simultaneous detection of the shrimp species Fenneropenaeus chinensis, Litopenaeus vannamei, and Penaeus monodon. / Lee, You Won; Lee, Seung Ha; Xin, Chun Feng; Shin, Ji Hun; Shin, Eunhee.

In: Journal of AOAC International, Vol. 100, No. 1, 01.01.2017, p. 104-108.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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AU - Shin, Ji Hun

AU - Shin, Eunhee

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AB - Due to mixed use of shrimp in foodstuffs, it is important to distinguish Fenneropenaeus chinensis from other shrimp species, such as Litopenaeus vannamei and Penaeus monodon. For this purpose, we have developed a multiplex PCR method to detect the simultaneous presence of all three species. Universal primers specific to the 16S ribosomal RNA subunit were used to sequence and ascertain the species in the samples. In this study, specific primers were designed to result in a unique PCR product size (143, 260, and 348 bp for L. vannamei, F. chinensis and P. monodon, respectively). In addition, duplex and triplex PCR protocols were developed to concurrently and rapidly detect these three shrimp species without nonspecific gene amplification. This multiplex PCR system is expected to be widely used to distinguish shrimp species that should be monitored to prevent their mixed use in food.

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