뇌척수액에서 실시간-이중-역전사중합효소연쇄반응을 이용한 장바이러스의 검출

Translated title of the contribution: Detection of enterovirus in cerebrospinal fluid by real-time nested reverse transcription polymerase chain reaction.

Se Ran Heo, Sun Kyung Jin, Ho Eun Chang, Kyoung Un Park, Junghan Song, Eui Chong Kim

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Enterovirus is a common cause of aseptic meningitis, respiratory disease and nonspecific febrile illness. The conventional methods for laboratory diagnosis of enterovirus infections have been virus culture and serotyping by an immunofluorecent test. We studied a new and more rapid approach for enterovirus detection in cerebrospinal fluid (CSF) by real-time nested PCR.

METHODS: This study was performed on 50 CSF specimens from patients suspected of aseptic meningitis. Enterovirus was detected in CSF by PCRs for 3 different targets and real-time nested PCR. Enterovirus culture was also performed in 44 CSF specimens.

RESULTS: The positive rate of PCRs for each of the 3 different targets was 26.0%, 40.0%, or 46.0%, and that of real-time nested PCR was 86.0%. Only 6.8% were positive in culture. Thus, the positive rate of real-time nested PCR was much higher than other methods.

CONCLUSIONS: Our study revealed that the real-time nested PCR should be useful for diagnosis of enterovirus infections because of a high sensitivity and rapid detection.

Original languageKorean
Pages (from-to)9-13
Number of pages5
JournalThe Korean journal of laboratory medicine
Volume26
Issue number1
StatePublished - Feb 2006

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Cerebrospinal fluid
Enterovirus
Polymerase chain reaction
Transcription
Reverse Transcription
Cerebrospinal Fluid
Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Enterovirus Infections
Aseptic Meningitis
Pulmonary diseases
Viruses
Serotyping
Clinical Laboratory Techniques
Fever

Cite this

@article{2ba8166519f0423e84b6b1300ef35215,
title = "뇌척수액에서 실시간-이중-역전사중합효소연쇄반응을 이용한 장바이러스의 검출",
abstract = "BACKGROUND: Enterovirus is a common cause of aseptic meningitis, respiratory disease and nonspecific febrile illness. The conventional methods for laboratory diagnosis of enterovirus infections have been virus culture and serotyping by an immunofluorecent test. We studied a new and more rapid approach for enterovirus detection in cerebrospinal fluid (CSF) by real-time nested PCR.METHODS: This study was performed on 50 CSF specimens from patients suspected of aseptic meningitis. Enterovirus was detected in CSF by PCRs for 3 different targets and real-time nested PCR. Enterovirus culture was also performed in 44 CSF specimens.RESULTS: The positive rate of PCRs for each of the 3 different targets was 26.0{\%}, 40.0{\%}, or 46.0{\%}, and that of real-time nested PCR was 86.0{\%}. Only 6.8{\%} were positive in culture. Thus, the positive rate of real-time nested PCR was much higher than other methods.CONCLUSIONS: Our study revealed that the real-time nested PCR should be useful for diagnosis of enterovirus infections because of a high sensitivity and rapid detection.",
author = "Heo, {Se Ran} and Jin, {Sun Kyung} and Chang, {Ho Eun} and Park, {Kyoung Un} and Junghan Song and Kim, {Eui Chong}",
year = "2006",
month = "2",
language = "Korean",
volume = "26",
pages = "9--13",
journal = "The Korean journal of laboratory medicine",
issn = "1598-6535",
publisher = "Seoul National University",
number = "1",

}

뇌척수액에서 실시간-이중-역전사중합효소연쇄반응을 이용한 장바이러스의 검출. / Heo, Se Ran; Jin, Sun Kyung; Chang, Ho Eun; Park, Kyoung Un; Song, Junghan; Kim, Eui Chong.

In: The Korean journal of laboratory medicine, Vol. 26, No. 1, 02.2006, p. 9-13.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 뇌척수액에서 실시간-이중-역전사중합효소연쇄반응을 이용한 장바이러스의 검출

AU - Heo, Se Ran

AU - Jin, Sun Kyung

AU - Chang, Ho Eun

AU - Park, Kyoung Un

AU - Song, Junghan

AU - Kim, Eui Chong

PY - 2006/2

Y1 - 2006/2

N2 - BACKGROUND: Enterovirus is a common cause of aseptic meningitis, respiratory disease and nonspecific febrile illness. The conventional methods for laboratory diagnosis of enterovirus infections have been virus culture and serotyping by an immunofluorecent test. We studied a new and more rapid approach for enterovirus detection in cerebrospinal fluid (CSF) by real-time nested PCR.METHODS: This study was performed on 50 CSF specimens from patients suspected of aseptic meningitis. Enterovirus was detected in CSF by PCRs for 3 different targets and real-time nested PCR. Enterovirus culture was also performed in 44 CSF specimens.RESULTS: The positive rate of PCRs for each of the 3 different targets was 26.0%, 40.0%, or 46.0%, and that of real-time nested PCR was 86.0%. Only 6.8% were positive in culture. Thus, the positive rate of real-time nested PCR was much higher than other methods.CONCLUSIONS: Our study revealed that the real-time nested PCR should be useful for diagnosis of enterovirus infections because of a high sensitivity and rapid detection.

AB - BACKGROUND: Enterovirus is a common cause of aseptic meningitis, respiratory disease and nonspecific febrile illness. The conventional methods for laboratory diagnosis of enterovirus infections have been virus culture and serotyping by an immunofluorecent test. We studied a new and more rapid approach for enterovirus detection in cerebrospinal fluid (CSF) by real-time nested PCR.METHODS: This study was performed on 50 CSF specimens from patients suspected of aseptic meningitis. Enterovirus was detected in CSF by PCRs for 3 different targets and real-time nested PCR. Enterovirus culture was also performed in 44 CSF specimens.RESULTS: The positive rate of PCRs for each of the 3 different targets was 26.0%, 40.0%, or 46.0%, and that of real-time nested PCR was 86.0%. Only 6.8% were positive in culture. Thus, the positive rate of real-time nested PCR was much higher than other methods.CONCLUSIONS: Our study revealed that the real-time nested PCR should be useful for diagnosis of enterovirus infections because of a high sensitivity and rapid detection.

M3 - Article

C2 - 18156692

VL - 26

SP - 9

EP - 13

JO - The Korean journal of laboratory medicine

JF - The Korean journal of laboratory medicine

SN - 1598-6535

IS - 1

ER -