Deregulation of DNA damage signal transduction by herpesvirus latency-associated M2

Xiaozhen Liang, Mary T. Pickering, Nam Hyuk Cho, Heesoon Chang, Michael R. Volkert, Timothy F. Kowalik, Jae U. Jung

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Infected cells recognize viral replication as a DNA damage stress and elicit a DNA damage response that ultimately induces apoptosis as part of host immune surveillance. Here, we demonstrate a novel mechanism where the marine gamma herpesvirus 68 (γHV68) latency-associated, anti-interferon M2 protein inhibits DNA damage-induced apoptosis by interacting with the DDB1/COP9/cullin repair complex and the ATM DNA damage signal transducer. M2 expression constitutively induced DDB1 nuclear localization and ATM kinase activation in the absence of DNA damage. Activated ATM subsequently induced Chk activation and p53 phosphorylation and stabilization without eliciting H2AX phosphorylation and MRN recruitment to foci upon DNA damage. Consequently, M2 expression inhibited DNA repair, rendered cells resistant to DNA damage-induced apoptosis, and induced a G1 cell cycle arrest. Our results suggest that γHV68 M2 blocks apoptosis-mediated intracellular innate immunity, which might ultimately contribute to its role in latent infection.

Original languageEnglish
Pages (from-to)5862-5874
Number of pages13
JournalJournal of Virology
Volume80
Issue number12
DOIs
StatePublished - Jun 2006

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