Delayed allograft rejection by the suppression of class II transactivator

Woon Kim Tae, Mi Choi Young, Nam Seo Jae, Hyun Kim Ju, Young Ho Suh, Doohyun Chung, Kyeong Cheon Jung, Ik Oh Kwon

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Abstract

We examined the effect of class II transactivator (CIITA) down-modulation on allograft rejection. To inhibit the function of CIITA, we constructed a series of CIITA mutants and found one exhibiting the dominant-negative effect on the regulation of major histocompatibility complex (MHC) class II expression. To test whether the CIITA dominant-negative mutant reduces immunogenecity, CIITA-transfected melanoma cells were injected into allogeneic host and assessed for immune evading activity against host immune cells. We demonstrated that the CIITA dominant-negative mutant allowed tumor nodules to develop earlier in the lung than control by this tumor challenge study. Furthermore, skin grafts deficient for CIITA also survived longer than wild-type in allogeneic hosts. Both the tumor challenge and skin graft studies suggest the inhibition of CIITA molecules in donor tissue would be beneficial to the control of allo-response.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalExperimental and Molecular Medicine
Volume38
Issue number3
StatePublished - 30 Jun 2006

Fingerprint

Allografts
Tumors
Grafts
Skin
Transplants
Neoplasms
MHC class II transactivator protein
Major Histocompatibility Complex
Melanoma
Modulation
Tissue Donors
Tissue
Lung
Molecules

Keywords

  • Graft rejection
  • Melanoma, experimental
  • MHC class II transactivator protein
  • Neoplasm transplantation
  • Transplantation, homologous

Cite this

Tae, W. K., Young, M. C., Jae, N. S., Ju, H. K., Suh, Y. H., Chung, D., ... Kwon, I. O. (2006). Delayed allograft rejection by the suppression of class II transactivator. Experimental and Molecular Medicine, 38(3), 210-216.
Tae, Woon Kim ; Young, Mi Choi ; Jae, Nam Seo ; Ju, Hyun Kim ; Suh, Young Ho ; Chung, Doohyun ; Jung, Kyeong Cheon ; Kwon, Ik Oh. / Delayed allograft rejection by the suppression of class II transactivator. In: Experimental and Molecular Medicine. 2006 ; Vol. 38, No. 3. pp. 210-216.
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Delayed allograft rejection by the suppression of class II transactivator. / Tae, Woon Kim; Young, Mi Choi; Jae, Nam Seo; Ju, Hyun Kim; Suh, Young Ho; Chung, Doohyun; Jung, Kyeong Cheon; Kwon, Ik Oh.

In: Experimental and Molecular Medicine, Vol. 38, No. 3, 30.06.2006, p. 210-216.

Research output: Contribution to journalArticleResearchpeer-review

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