COX-2 regulates p53 activity and inhibits DNA damage-induced apoptosis

Eun Mi Choi, Jee In Heo, Jin Young Oh, Young Myeong Kim, Kwon Soo Ha, Jong-Il Kim, Jeong A. Han

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Abstract

We have previously shown that p53 induces cyclooxygenase-2 (COX-2) expression and COX-2 inhibits p53- or genotoxic stress-induced apoptosis. However, the COX-2 effects have been demonstrated indirectly by the use of a selective inhibitor, NS-398, and the molecular mechanisms by which COX-2 inhibits apoptosis have not been identified. In the present study, we demonstrated that COX-2 inhibits genotoxic stress-induced apoptosis by using an adenoviral COX-2 overexpression system. In addition, we found that COX-2 regulates the transcription function of p53 as evidenced by suppression of p53 target gene induction by COX-2 cotransfection. Furthermore, COX-2 interacted with p53 in vitro and in vivo, which was inhibited by the treatment with NS-398. Taken together, these results suggest a novel function of COX-2 that inhibits DNA damage-induced apoptosis through direct regulation of p53 function.

Original languageEnglish
Pages (from-to)1107-1112
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume328
Issue number4
DOIs
StatePublished - 25 Mar 2005

Fingerprint

Cyclooxygenase 2
DNA Damage
Apoptosis
DNA
p53 Genes
Transcription
Genes

Keywords

  • Apoptosis
  • COX-2
  • Interaction
  • NS-398
  • Overexpression
  • Transcription
  • p53

Cite this

Choi, Eun Mi ; Heo, Jee In ; Oh, Jin Young ; Kim, Young Myeong ; Ha, Kwon Soo ; Kim, Jong-Il ; Han, Jeong A. / COX-2 regulates p53 activity and inhibits DNA damage-induced apoptosis. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 328, No. 4. pp. 1107-1112.
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COX-2 regulates p53 activity and inhibits DNA damage-induced apoptosis. / Choi, Eun Mi; Heo, Jee In; Oh, Jin Young; Kim, Young Myeong; Ha, Kwon Soo; Kim, Jong-Il; Han, Jeong A.

In: Biochemical and Biophysical Research Communications, Vol. 328, No. 4, 25.03.2005, p. 1107-1112.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Choi, Eun Mi

AU - Heo, Jee In

AU - Oh, Jin Young

AU - Kim, Young Myeong

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