Contribution of SLC22A12 on hypouricemia and its clinical significance for screening purposes

Do Hyeon Cha, Heon Yung Gee, Raul Cachau, Jong Mun Choi, Daeui Park, Sun Ha Jee, Seungho Ryu, Kyeong Kyu Kim, Hong Hee Won, Sophie Limou, Woo Jae Myung, Cheryl A. Winkler, Sung Kweon Cho

Research output: Contribution to journalArticle

Abstract

Differentiating between inherited renal hypouricemia and transient hypouricemic status is challenging. Here, we aimed to describe the genetic background of hypouricemia patients using whole-exome sequencing (WES) and assess the feasibility for genetic diagnosis using two founder variants in primary screening. We selected all cases (N = 31) with extreme hypouricemia (<1.3 mg/dl) from a Korean urban cohort of 179,381 subjects without underlying conditions. WES and corresponding downstream analyses were performed for the discovery of rare causal variants for hypouricemia. Two known recessive variants within SLC22A12 (p.Trp258*, pArg90His) were identified in 24 out of 31 subjects (77.4%). In an independent cohort, we identified 50 individuals with hypouricemia and genotyped the p.Trp258* and p.Arg90His variants; 47 of the 50 (94%) hypouricemia cases were explained by only two mutations. Four novel coding variants in SLC22A12, p.Asn136Lys, p.Thr225Lys, p.Arg284Gln, and p.Glu429Lys, were additionally identified. In silico studies predict these as pathogenic variants. This is the first study to show the value of genetic diagnostic screening for hypouricemia in the clinical setting. Screening of just two ethnic-specific variants (p.Trp258* and p.Arg90His) identified 87.7% (71/81) of Korean patients with monogenic hypouricemia. Early genetic identification of constitutive hypouricemia may prevent acute kidney injury by avoidance of dehydration and excessive exercise.

Original languageEnglish
Article number14360
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - 1 Dec 2019

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Exome
Genetic Testing
Dehydration
Acute Kidney Injury
Computer Simulation
Exercise
Mutation
Genetic Background
Renal hypouricemia

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Cha, D. H., Gee, H. Y., Cachau, R., Choi, J. M., Park, D., Jee, S. H., ... Cho, S. K. (2019). Contribution of SLC22A12 on hypouricemia and its clinical significance for screening purposes. Scientific Reports, 9(1), [14360]. https://doi.org/10.1038/s41598-019-50798-6
Cha, Do Hyeon ; Gee, Heon Yung ; Cachau, Raul ; Choi, Jong Mun ; Park, Daeui ; Jee, Sun Ha ; Ryu, Seungho ; Kim, Kyeong Kyu ; Won, Hong Hee ; Limou, Sophie ; Myung, Woo Jae ; Winkler, Cheryl A. ; Cho, Sung Kweon. / Contribution of SLC22A12 on hypouricemia and its clinical significance for screening purposes. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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Cha, DH, Gee, HY, Cachau, R, Choi, JM, Park, D, Jee, SH, Ryu, S, Kim, KK, Won, HH, Limou, S, Myung, WJ, Winkler, CA & Cho, SK 2019, 'Contribution of SLC22A12 on hypouricemia and its clinical significance for screening purposes', Scientific Reports, vol. 9, no. 1, 14360. https://doi.org/10.1038/s41598-019-50798-6

Contribution of SLC22A12 on hypouricemia and its clinical significance for screening purposes. / Cha, Do Hyeon; Gee, Heon Yung; Cachau, Raul; Choi, Jong Mun; Park, Daeui; Jee, Sun Ha; Ryu, Seungho; Kim, Kyeong Kyu; Won, Hong Hee; Limou, Sophie; Myung, Woo Jae; Winkler, Cheryl A.; Cho, Sung Kweon.

In: Scientific Reports, Vol. 9, No. 1, 14360, 01.12.2019.

Research output: Contribution to journalArticle

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AU - Gee, Heon Yung

AU - Cachau, Raul

AU - Choi, Jong Mun

AU - Park, Daeui

AU - Jee, Sun Ha

AU - Ryu, Seungho

AU - Kim, Kyeong Kyu

AU - Won, Hong Hee

AU - Limou, Sophie

AU - Myung, Woo Jae

AU - Winkler, Cheryl A.

AU - Cho, Sung Kweon

PY - 2019/12/1

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