Constitutively active H-ras accelerates multiple forms of plasticity in developing visual cortex

Megumi Kaneko, Claire E. Cheetham, Yong-Seok Lee, Alcino J. Silva, Michael P. Stryker, Kevin Fox

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

Experience-dependent cortical plasticity has been studied by using loss-of-function methods. Here, we take the complementary approach of using a genetic gain-of-function that enhances plasticity. We show that a constitutively active form of H-ras (H-rasG12V), expressed presynaptically at excitatory synapses in mice, accelerates and enhances multiple, mechanistically distinct forms of plasticity in the developing visual cortex. In vivo, H-rasG12V not only increased the rate of ocular dominance change in response to monocular deprivation (MD), but also accelerated recovery from deprivation by reverse occlusion. In vitro, H-rasG12V expression decreased baseline presynaptic release probability and enhanced presynaptically expressed longterm potentiation(LTP). H-rasG12V expression alsoaccelerated the increase following MD in the frequency of miniature excita tory potentials, mirroring accelerated plasticity in vivo. These findings demonstrate accelerated neocortical plasticity, which offers an avenue toward future therapies for many neurological and neuropsychiatric disorders.

Original languageEnglish
Pages (from-to)19026-19031
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number44
DOIs
StatePublished - 2 Nov 2010

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ocular Dominance
Visual Cortex
Nervous System Diseases
Synapses
Therapeutics
In Vitro Techniques

Keywords

  • Again of function
  • Monocular deprivation
  • Mouse
  • Ocular dominance
  • Presynaptic long-term potentiation

Cite this

Kaneko, Megumi ; Cheetham, Claire E. ; Lee, Yong-Seok ; Silva, Alcino J. ; Stryker, Michael P. ; Fox, Kevin. / Constitutively active H-ras accelerates multiple forms of plasticity in developing visual cortex. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 44. pp. 19026-19031.
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Constitutively active H-ras accelerates multiple forms of plasticity in developing visual cortex. / Kaneko, Megumi; Cheetham, Claire E.; Lee, Yong-Seok; Silva, Alcino J.; Stryker, Michael P.; Fox, Kevin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 44, 02.11.2010, p. 19026-19031.

Research output: Contribution to journalArticleResearchpeer-review

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